Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/114
Título: The multigenic structure of the MHC locus contributes to positive selection efficiency: A role for MHC class II gene-genespecific
Autor: Monteiro, Miguel Caetano
Couceiro, Sofia
Penha-Gonçalves, Carlos
Palavras-chave: MHC
Multigenic
Repertoire development
Thymic selection
Data: 2005
Editora: Wiley
Citação: Monteiro, M. C., Couceiro S., Penha-Gonçalves C. (2013). The multigenic structure of the MHC locus contributes to positive selection efficiency: a role for MHC class II gene-specific restriction. European Journal of Immunology. 35(12): 1-10
Resumo: The study of T cell positive selection in the thymus has long been focused on the specificity of the MHC-TCR interactions, making use of genetically manipulated mice that display TCR specificities or selecting peptides of limited diversity. However, little is known on the role of the MHC molecules irrespective of the peptide specificity and the implications of MHC multigenic structure in thymic positive selection have not been addressed. Here, we investigated the effect of MHC class II genetic configuration on the positive selection efficiency of naturally generated pre-selection repertoires in the mouse thymus. Analysis of positively selected thymocyte populations in MHC-congenic and -transgenic mice revealed that expression of I-E molecule in the thymic cortex increases positive selection efficiency of CD4 cells by approximately 50%.We show that increments in positive selection attributable to either the I-A and I-E genes are not due to increased MHC class II expression in the thymic cortex and are not affected by the number of MHC alleles. Collectively, our findings imply that MHC class II generestricted TCR specificities significantly contribute to positive selection efficiency, introducing the notion that multigenic structure of the MHC locus serves to increase selection of non-overlapping TCR repertoires.
Peer review: Yes
URI: http://hdl.handle.net/10400.7/114
ISSN: 1521-4141
Versão do Editor: http://dx.doi.org/10.1002/eji.200535190
Aparece nas colecções:DG - Artigos em revistas científicas

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