Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/195
Título: Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
Autor: Beldade, P.
Van´T Hof, Arje`n E.
Jerónimo, MA.
Long, AD.
Palavras-chave: Bicyclus anynana
EST
Genetic markers
Lepidoptera
Microsatellite
Polymorphism
Data: Fev-2009
Editora: Blackwell
Citação: Beldade P, A van't Hof, MA Jerónimo & AD Long. 2009. Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies. Molecular Ecology Resources 9,1487-1492
Resumo: Deriving useful microsatellite markers in lepidopterans has been challenging when relying on scans of genomic DNA libraries, presumably due to repetitiveness in their genomes. We assayed 96 of 320 microsatellites identified in silico from a collection of Bicyclus anynana ESTs, in 11 independent individuals from a laboratory population.From the 68 successful assays, we identified 40 polymorphic markers including 22 with BLAST-based annotation. Nine of 12 selected polymorphic markers tested in a panel of 24 wild-caught individuals converted to successful assays and were all polymorphic. We discuss how microsatellite discovery in ESTs is an efficient strategy with important attendant advantages.
URI: http://hdl.handle.net/10400.7/195
ISSN: 1755-098X
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