We study how chromosome architecture contributes to faithful genome segregation. Genome stability relies on the fact that at each round of cell division, the genetic information encoded in the DNA molecules is properly segregated into the two daughter cells. Proper completion of this process, in turn, depends on two major changes in chromosome organization: 1) The two-sister DNA molecules remain tightly associated with each other from the moment of DNA replication until the later stages of the subsequent mitosis; 2) At the onset of nuclear division, chromatin is converted into compact structures with the right mechanical properties (size, flexibility, and rigidity) to facilitate their segregation. Our laboratory adopts a multidisciplinary approach, combining Drosophila genetics, acute protein inactivation, 4D-live cell imaging and biophysical/mathematical modelling to evaluate how dynamic mitotic chromosomes are assembled and how their morphology influences the mechanical aspects of chromosome movement and cell cycle checkpoint signalling. In parallel we aim to dissect how different cells respond to compromised chromosome cohesion and condensation, both at the cellular and organism level. By studying the contribution of chromosome structure in the mechanics of nuclear division we aim to identify novel routes to aneuploidy that underlie several human conditions, including developmental diseases, cancer and infertility.