Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/340
Título: The Toll-dorsal pathway is required for resistance to viral oral infection in Drosophila
Autor: Ferreira, Álvaro Gil
Naylor, Huw
Esteves, Sara Santana
Pais, Inês Silva
Martins, Nelson Eduardo
Teixeira, Luis
Palavras-chave: Drosophila melanogaster
viral transmission and infection
antibodies
Data: 4-Dez-2014
Editora: PLOS
Citação: Ferreira A ́ G, Naylor H, Esteves SS, Pais IS, Martins NE, et al. (2014) The Toll-Dorsal Pathway Is Required for Resistance to Viral Oral Infection in Drosophila . PLoS Pathog 10(12): e1004507. doi:10.1371/journal.ppat.1004507
Resumo: Pathogen entry route can have a strong impact on the result of microbial infections in different hosts, including insects. Drosophila melanogaster has been a successful model system to study the immune response to systemic viral infection. Here we investigate the role of the Toll pathway in resistance to oral viral infection in D. melanogaster. We show that several Toll pathway components, including Spätzle, Toll, Pelle and the NF-kB-like transcription factor Dorsal, are required to resist oral infection with Drosophila C virus. Furthermore, in the fat body Dorsal is translocated from the cytoplasm to the nucleus and a Toll pathway target gene reporter is upregulated in response to Drosophila C Virus infection. This pathway also mediates resistance to several other RNA viruses (Cricket paralysis virus, Flock House virus, and Nora virus). Compared with control, viral titres are highly increased in Toll pathway mutants. The role of the Toll pathway in resistance to viruses in D. melanogaster is restricted to oral infection since we do not observe a phenotype associated with systemic infection. We also show that Wolbachia and other Drosophila-associated microbiota do not interact with the Toll pathway-mediated resistance to oral infection. We therefore identify the Toll pathway as a new general inducible pathway that mediates strong resistance to viruses with a route-specific role. These results contribute to a better understanding of viral oral infection resistance in insects, which is particularly relevant in the context of transmission of arboviruses by insect vectors.
Peer review: yes
URI: http://hdl.handle.net/10400.7/340
DOI: 10.1371/journal.ppat.1004507
10.1371/journal.ppat.1004507
10.1371/journal.ppat.1004507
Versão do Editor: http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1004507
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