Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/356
Título: V-ATPase Proton Pumping Activity Is Required for Adult Zebrafish Appendage Regeneration
Autor: Monteiro, Joana
Aires, Rita
Becker, Jörg D.
Jacinto, António
Certal, Ana C.
Rodríguez-León, Joaquín
Data: 26-Mar-2014
Editora: PLOS
Citação: Monteiro J, Aires R, Becker JD, Jacinto A, Certal AC, Rodríguez-León J (2014) V-ATPase Proton Pumping Activity Is Required for Adult Zebrafish Appendage Regeneration. PLoS ONE 9(3): e92594. doi:10.1371/journal.pone.0092594
Resumo: The activity of ion channels and transporters generates ion-specific fluxes that encode electrical and/or chemical signals with biological significance. Even though it is long known that some of those signals are crucial for regeneration, only in recent years the corresponding molecular sources started to be identified using mainly invertebrate or larval vertebrate models. We used adult zebrafish caudal fin as a model to investigate which and how ion transporters affect regeneration in an adult vertebrate model. Through the combined use of biophysical and molecular approaches, we show that V-ATPase activity contributes to a regeneration-specific H+ ef`flux. The onset and intensity of both V-ATPase expression and H+ efflux correlate with the different regeneration rate along the proximal-distal axis. Moreover, we show that V-ATPase inhibition impairs regeneration in adult vertebrate. Notably, the activity of this H+ pump is necessary for aldh1a2 and mkp3 expression, blastema cell proliferation and fin innervation. To the best of our knowledge, this is the first report on the role of V-ATPase during adult vertebrate regeneration.
Peer review: yes
URI: http://hdl.handle.net/10400.7/356
DOI: 10.1371/journal.pone.0092594
10.1371/journal.pone.0092594
10.1371/journal.pone.0092594
Versão do Editor: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0092594
Aparece nas colecções:PG - Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
journal.pone.0092594.pdf1,26 MBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.