Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/357
Título: Host adaptation to viruses relies on few genes with different cross-resistance properties
Autor: Martins, Nelson E
Faria, Vítor G
Nolte, Viola
Schlötterer, Christian
Teixeira, Luis
Sucena, Élio
Magalhães, Sara
Palavras-chave: host–parasite interactions
experimental evolution
Drosophila immunity
evolve and resequence
Data: 22-Abr-2014
Editora: National Academy of Sciences
Citação: Nelson E. Martins, Vítor G. Faria, Viola Nolte, Christian Schlötterer, Luis Teixeira, Élio Sucena, and Sara Magalhães Host adaptation to viruses relies on few genes with different cross-resistance properties PNAS 2014 111 (16) 5938-5943; published ahead of print April 7, 2014, doi:10.1073/pnas.1400378111
Resumo: Host adaptation to one parasite may affect its response to others. However, the genetics of these direct and correlated responses remains poorly studied. The overlap between these responses is instrumental for the understanding of host evolution in multiparasite environments. We determined the genetic and phenotypic changes underlying adaptation of Drosophila melanogaster to Drosophila C virus (DCV). Within 20 generations, flies selected with DCV showed increased survival after DCV infection, but also after cricket paralysis virus (CrPV) and flock house virus (FHV) infection. Whole-genome sequencing identified two regions of significant differentiation among treatments, from which candidate genes were functionally tested with RNAi. Three genes were validated--pastrel, a known DCV-response gene, and two other loci, Ubc-E2H and CG8492. Knockdown of Ubc-E2H and pastrel also led to increased sensitivity to CrPV, whereas knockdown of CG8492 increased susceptibility to FHV infection. Therefore, Drosophila adaptation to DCV relies on few major genes, each with different cross-resistance properties, conferring host resistance to several parasites.
Peer review: yes
URI: http://hdl.handle.net/10400.7/357
DOI: 10.1073/pnas.1400378111
Versão do Editor: http://www.pnas.org/content/111/16/5938.long
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