Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/383
Título: Antioxidant and Antimicrobial Potential of the Bifurcaria bifurcata Epiphytic Bacteria
Autor: Horta, André
Pinteus, Susete
Alves, Celso
Fino, Nádia
Silva, Joana
Fernandez, Sara
Rodrigues, Américo
Pedrosa, Rui
Palavras-chave: marine microorganisms
natural compounds
human pathogenic
Alteromonas sp
Shewanella sp
antibacterial
surface-associated microorganisms
marine symbiosis
macro-algae
seaweeds
Data: 24-Mar-2014
Editora: MDPI
Citação: Horta, A.; Pinteus, S.; Alves, C.; Fino, N.; Silva, J.; Fernandez, S.; Rodrigues, A.; Pedrosa, R. Antioxidant and Antimicrobial Potential of the Bifurcaria bifurcata Epiphytic Bacteria. Mar. Drugs 2014, 12, 1676-1689.
Resumo: Surface-associated marine bacteria are an interesting source of new secondary metabolites. The aim of this study was the isolation and identification of epiphytic bacteria from the marine brown alga, Bifurcaria bifurcata, and the evaluation of the antioxidant and antimicrobial activity of bacteria extracts. The identification of epiphytic bacteria was determined by 16S rRNA gene sequencing. Bacteria extracts were obtained with methanol and dichloromethane (1:1) extraction. The antioxidant activity of extracts was performed by quantification of total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and oxygen radical absorbance capacity (ORAC). Antimicrobial activities were evaluated against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella enteritidis, Staphylococcus aureus, Saccharomyces cerevisiae and Candida albicans. A total of 39 Bifurcaria bifurcata-associated bacteria were isolated and 33 were identified as Vibrio sp. (48.72%), Alteromonas sp. (12.82%), Shewanella sp. (12.26%), Serratia sp. (2.56%), Citricoccus sp. (2.56%), Cellulophaga sp. (2.56%), Ruegeria sp. (2.56%) and Staphylococcus sp. (2.56%). Six (15.38%) of the 39 bacteria Bifurcaria bifurcata-associated bacteria presented less than a 90% Basic Local Alignment Search Tool (BLAST) match, and some of those could be new. The highest antioxidant activity and antimicrobial activity (against B. subtilis) was exhibited by strain 16 (Shewanella sp.). Several strains also presented high antimicrobial activity against S. aureus, mainly belonging to Alteromonas sp. and Vibrio sp. There were no positive results against fungi and Gram-negative bacteria. Bifurcaria bifurcata epiphytic bacteria were revealed to be excellent sources of natural antioxidant and antimicrobial compounds.
Descrição: This article belongs to the Special Issue Selected Papers from the 14th International Symposium on Marine Natural Products
Peer review: yes
URI: http://hdl.handle.net/10400.7/383
DOI: 10.3390/md12031676
Versão do Editor: http://www.mdpi.com/1660-3397/12/3/1676
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