Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/384
Título: Cdk1 Restrains NHEJ through Phosphorylation of XRCC4-like Factor Xlf1
Autor: Hentges, Pierre
Waller, Helen
Reis, Clara C.
Ferreira, Miguel Godinho
Doherty, Aidan J.
Data: 24-Dez-2014
Editora: Cell Press
Resumo: Eukaryotic cells use two principal mechanisms for repairing DNA double-strand breaks (DSBs): homologous recombination (HR) and nonhomologous end-joining (NHEJ). DSB repair pathway choice is strongly regulated during the cell cycle. Cyclin-dependent kinase 1 (Cdk1) activates HR by phosphorylation of key recombination factors. However, a mechanism for regulating the NHEJ pathway has not been established. Here, we report that Xlf1, a fission yeast XLF ortholog, is a key regulator of NHEJ activity in the cell cycle. We show that Cdk1 phosphorylates residues in the C terminus of Xlf1 over the course of the cell cycle. Mutation of these residues leads to the loss of Cdk1 phosphorylation, resulting in elevated levels of NHEJ repair in vivo. Together, these data establish that Xlf1 phosphorylation by Cdc2(Cdk1) provides a molecular mechanism for downregulation of NHEJ in fission yeast and indicates that XLF is a key regulator of end-joining processes in eukaryotic organisms.
Peer review: yes
URI: http://hdl.handle.net/10400.7/384
DOI: 10.1016/j.celrep.2014.11.044
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S2211124714010109
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