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|Título:||A Genomic Signature and the Identification of New Sporulation Genes|
|Autor:||Abecasis, A. B.|
Pereira-Leal, J. B.
Henriques, A. O.
|Editora:||American Society for Microbiology|
|Citação:||A Genomic Signature and the Identification of New Sporulation Genes Ana B. Abecasis, Mónica Serrano, Renato Alves, Leonor Quintais, José B. Pereira-Leal, and Adriano O. Henriques J. Bacteriol. May 2013 195:9 2101-2115; Accepted manuscript posted online 8 February 2013, doi:10.1128/JB.02110-12|
|Resumo:||Bacterial endospores are the most resistant cell type known to humans, as they are able to withstand extremes of temperature, pressure, chemical injury, and time. They are also of interest because the endospore is the infective particle in a variety of human and livestock diseases. Endosporulation is characterized by the morphogenesis of an endospore within a mother cell. Based on the genes known to be involved in endosporulation in the model organism Bacillus subtilis, a conserved core of about 100 genes was derived, representing the minimal machinery for endosporulation. The core was used to define a genomic signature of about 50 genes that are able to distinguish endospore-forming organisms, based on complete genome sequences, and we show this 50-gene signature is robust against phylogenetic proximity and other artifacts. This signature includes previously uncharacterized genes that we can now show are important for sporulation in B. subtilis and/or are under developmental control, thus further validating this genomic signature. We also predict that a series of polyextremophylic organisms, as well as several gut bacteria, are able to form endospores, and we identified 3 new loci essential for sporulation in B. subtilis: ytaF, ylmC, and ylzA. In all, the results support the view that endosporulation likely evolved once, at the base of the Firmicutes phylum, and is unrelated to other bacterial cell differentiation programs and that this involved the evolution of new genes and functions, as well as the cooption of ancestral, housekeeping functions.|
|Versão do Editor:||http://jb.asm.org/content/195/9/2101.long|
|Aparece nas colecções:||CG - Artigos|
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|J. Bacteriol.-2013-Abecasis-2101-15.pdf||artigo principal||2,53 MB||Adobe PDF||Ver/Abrir|
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