Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/423
Título: Nucleozin Targets Cytoplasmic Trafficking of Viral Ribonucleoprotein-Rab11 Complexes in Influenza A Virus Infection
Autor: Amorim, M. J.
Kao, R. Y.
Digard, P.
Palavras-chave: Ribonucleoproteins
Influenza A virus
Infection
Data: 2013
Editora: American Society for Microbiology
Citação: Nucleozin Targets Cytoplasmic Trafficking of Viral Ribonucleoprotein-Rab11 Complexes in Influenza A Virus Infection Maria Joao Amorim, Richard Y. Kao, and Paul Digard J. Virol. April 2013 87:8 4694-4703; Accepted manuscript posted online 13 February 2013, doi:10.1128/JVI.03123-12
Resumo: Novel antivirals are needed to supplement existing control strategies for influenza A virus (IAV). A promising new class of drug, exemplified by the compound nucleozin, has recently been identified that targets the viral nucleoprotein (NP). These inhibitors are thought to act as "molecular staples" that stabilize interactions between NP monomers, promoting the formation of nonfunctional aggregates. Here we detail the inhibitory mechanism of nucleozin, finding that the drug has both early- and late-acting effects on the IAV life cycle. When present at the start of infection, it inhibited viral RNA and protein synthesis. However, when added at later time points, it still potently blocked the production of infectious progeny but without affecting viral macromolecular synthesis. Instead, nucleozin blocked the cytoplasmic trafficking of ribonucleoproteins (RNPs) that had undergone nuclear export, promoting the formation of large perinuclear aggregates of RNPs along with cellular Rab11. This effect led to the production of much reduced amounts of often markedly smaller virus particles. We conclude that the primary target of nucleozin is the viral RNP, not NP, and this work also provides proof of the principle that IAV replication can be effectively inhibited by blocking cytoplasmic trafficking of the viral genome.
Peer review: yes
URI: http://hdl.handle.net/10400.7/423
DOI: 10.1128/JVI.03123-12
Versão do Editor: http://jvi.asm.org/content/87/8/4694.long
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