Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/447
Título: Sialoglycoproteins and N-Glycans from Secreted Exosomes of Ovarian Carcinoma Cells
Autor: Escrevente, Cristina
Grammel, Nicolas
Kandzia, Sebastian
Zeiser, Johannes
Tranfield, Erin M.
Conradt, Harald S.
Costa, Júlia
Palavras-chave: Sialoglycoproteins
Ovarian Neoplasms
Cell Line, Tumor
Tumor Markers, Biological
Data: 24-Out-2013
Editora: PLOS
Citação: Escrevente C, Grammel N, Kandzia S, Zeiser J, Tranfield EM, et al. (2013) Sialoglycoproteins and N-Glycans from Secreted Exosomes of Ovarian Carcinoma Cells. PLoS ONE 8(10): e78631. doi:10.1371/journal.pone.0078631
Resumo: Exosomes consist of vesicles that are secreted by several human cells, including tumor cells and neurons, and they are found in several biological fluids. Exosomes have characteristic protein and lipid composition, however, the results concerning glycoprotein composition and glycosylation are scarce. Here, protein glycosylation of exosomes from ovarian carcinoma SKOV3 cells has been studied by lectin blotting, NP-HPLC analysis of 2-aminobenzamide labeled glycans and mass spectrometry. An abundant sialoglycoprotein was found enriched in exosomes and it was identified by peptide mass fingerprinting and immunoblot as the galectin-3-binding protein (LGALS3BP). Exosomes were found to contain predominantly complex glycans of the di-, tri-, and tetraantennary type with or without proximal fucose and also high mannose glycans. Diantennary glycans containing bisecting N-acetylglucosamine were also detected. This work provides detailed information about glycoprotein and N-glycan composition of exosomes from ovarian cancer cells, furthermore it opens novel perspectives to further explore the functional role of glycans in the biology of exosomes.
Peer review: yes
URI: http://hdl.handle.net/10400.7/447
DOI: 10.1371/journal.pone.0078631
Versão do Editor: http://www.plosone.org/article/Authors/info:doi/10.1371/journal.pone.0078631
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