Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/451
Título: Role of a polymorphism in a Hox/Pax-responsive enhancer in the evolution of the vertebrate spine
Autor: Guerreiro, Isabel
Nunes, Andreia
Woltering, Joost M
Casaca, Ana
Nóvoa, Ana
Vinagre, Tânia
Hunter, Margaret E
Duboule, Denis
Mallo, Moisés
Palavras-chave: Polymorphism, Single Nucleotide
Enhancer Elements, Genetic
Spine
Data: 25-Jun-2013
Editora: National Academy of Sciences
Citação: Isabel Guerreiro, Andreia Nunes, Joost M. Woltering, Ana Casaca, Ana Nóvoa, Tânia Vinagre, Margaret E. Hunter, Denis Duboule, and Moisés Mallo Role of a polymorphism in a Hox/Pax-responsive enhancer in the evolution of the vertebrate spine PNAS 2013 110 (26) 10682-10686; published ahead of print May 14, 2013, doi:10.1073/pnas.1300592110
Resumo: Patterning of the vertebrate skeleton requires the coordinated activity of Hox genes. In particular, Hox10 proteins are essential to set the transition from thoracic to lumbar vertebrae because of their rib-repressing activity. In snakes, however, the thoracic region extends well into Hox10-expressing areas of the embryo, suggesting that these proteins are unable to block rib formation. Here, we show that this is not a result of the loss of rib-repressing properties by the snake proteins, but rather to a single base pair change in a Hox/Paired box (Pax)-responsive enhancer, which prevents the binding of Hox proteins. This polymorphism is also found in Paenungulata, such as elephants and manatees, which have extended rib cages. In vivo, this modified enhancer failed to respond to Hox10 activity, supporting its role in the extension of rib cages. In contrast, the enhancer could still interact with Hoxb6 and Pax3 to promote rib formation. These results suggest that a polymorphism in the Hox/Pax-responsive enhancer may have played a role in the evolution of the vertebrate spine by differently modulating its response to rib-suppressing and rib-promoting Hox proteins.
Peer review: yes
URI: http://hdl.handle.net/10400.7/451
DOI: 10.1073/pnas.1300592110
Versão do Editor: http://www.pnas.org/content/110/26/10682.abstract
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