Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/457
Título: Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules
Autor: Limpens, Erik
Moling, Sjef
Hooiveld, Guido
Pereira, Patrícia A.
Bisseling, Ton
Becker, Jörg D.
Küster, Helge
Palavras-chave: Cells
Gene Expression Profiling
Medicago truncatula
Root Nodules, Plant
Data: 29-Mai-2013
Editora: PLOS
Citação: Limpens E, Moling S, Hooiveld G, Pereira PA, Bisseling T, Becker JD, et al. (2013) Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules. PLoS ONE 8(5): e64377. doi:10.1371/journal.pone.0064377
Resumo: Legumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture microdissection. Next, we determined their associated expression profiles using Affymetrix Medicago GeneChips. Cells were collected from the nodule infection zone divided into a distal (where symbiosome formation and division occur) and proximal region (where symbiosomes are mainly differentiating), as well as infected cells from the fixation zone containing mature nitrogen fixing symbiosomes. As non-infected cells/tissue we included nodule meristem cells and uninfected cells from the fixation zone. Here, we present a comprehensive gene expression map of an indeterminate Medicago nodule and selected genes that show specific enriched expression in the different cells or tissues. Validation of the obtained expression profiles, by comparison to published gene expression profiles and experimental verification, indicates that the data can be used as digital "in situ". This digital "in situ" offers a genome-wide insight into genes specifically associated with subsequent stages of symbiosome and nodule cell development, and can serve to guide future functional studies.
Peer review: yes
URI: http://hdl.handle.net/10400.7/457
DOI: 10.1371/journal.pone.0064377
Versão do Editor: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0064377
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