Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/477
Título: Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila
Autor: Regan, Jennifer C.
Brandão, Ana S.
Leitão, Alexandre B.
Mantas Dias, Ângela Raquel
Sucena, Élio
Jacinto, António
Zaidman-Rémy, Anna
Palavras-chave: Drosophila melanogaster
Gene expression
Gene regulation
Hemocytes
Larvae
Metamorphosis
Metamorphosis
Phagocytosis
Pupae
Data: 24-Out-2013
Editora: PLOS
Citação: Regan JC, Branda˜o AS, Leita˜o AB, Mantas Dias Aˆ R, Sucena E´ , et al. (2013) Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila. PLoS Pathog 9(10): e1003720. doi:10.1371/journal.ppat.1003720
Resumo: Coupling immunity and development is essential to ensure survival despite changing internal conditions in the organism. Drosophila metamorphosis represents a striking example of drastic and systemic physiological changes that need to be integrated with the innate immune system. However, nothing is known about the mechanisms that coordinate development and immune cell activity in the transition from larva to adult. Here, we reveal that regulation of macrophage-like cells (hemocytes) by the steroid hormone ecdysone is essential for an effective innate immune response over metamorphosis. Although it is generally accepted that steroid hormones impact immunity in mammals, their action on monocytes (e.g. macrophages and neutrophils) is still not well understood. Here in a simpler model system, we used an approach that allows in vivo, cell autonomous analysis of hormonal regulation of innate immune cells, by combining genetic manipulation with flow cytometry, high-resolution time-lapse imaging and tissue-specific transcriptomic analysis. We show that in response to ecdysone, hemocytes rapidly upregulate actin dynamics, motility and phagocytosis of apoptotic corpses, and acquire the ability to chemotax to damaged epithelia. Most importantly, individuals lacking ecdysone-activated hemocytes are defective in bacterial phagocytosis and are fatally susceptible to infection by bacteria ingested at larval stages, despite the normal systemic and local production of antimicrobial peptides. This decrease in survival is comparable to the one observed in pupae lacking immune cells altogether, indicating that ecdysone-regulation is essential for hemocyte immune functions and survival after infection. Microarray analysis of hemocytes revealed a large set of genes regulated at metamorphosis by EcR signaling, among which many are known to function in cell motility, cell shape or phagocytosis. This study demonstrates an important role for steroid hormone regulation of immunity in vivo in Drosophila, and paves the way for genetic dissection of the mechanisms at work behind steroid regulation of innate immune cells.
Peer review: yes
URI: http://hdl.handle.net/10400.7/477
DOI: 10.1371/journal.ppat.1003720
Versão do Editor: http://www.plospathogens.org/article/Authors/info:doi/10.1371/journal.ppat.1003720
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