Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/478
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dc.contributor.authorde Rosa, M.-
dc.contributor.authorZacarias, S.-
dc.contributor.authorAthanasiadis, A.-
dc.date.accessioned2015-11-09T16:26:50Z-
dc.date.available2015-11-09T16:26:50Z-
dc.date.issued2013-07-26-
dc.identifier10.1093/nar/gkt743-
dc.identifier.citationMatteo de Rosa, Sonia Zacarias, and Alekos Athanasiadis Structural basis for Z-DNA binding and stabilization by the zebrafish Z-DNA dependent protein kinase PKZ Nucl. Acids Res. (2013) 41 (21): 9924-9933 first published online August 23, 2013 doi:10.1093/nar/gkt743pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/478-
dc.description.abstractThe RNA-dependent protein kinase PKR plays a central role in the antiviral defense of vertebrates by shutting down protein translation upon detection of viral dsRNA in the cytoplasm. In some teleost fish, PKZ, a homolog of PKR, performs the same function, but surprisingly, instead of dsRNA binding domains, it harbors two Z-DNA/Z-RNA-binding domains belonging to the Zalpha domain family. Zalpha domains have also been found in other proteins, which have key roles in the regulation of interferon responses such as ADAR1 and DNA-dependent activator of IFN-regulatory factors (DAI) and in viral proteins involved in immune response evasion such as the poxviral E3L and the Cyprinid Herpesvirus 3 ORF112. The underlying mechanism of nucleic acids binding and stabilization by Zalpha domains is still unclear. Here, we present two crystal structures of the zebrafish PKZ Zalpha domain (DrZalpha(PKZ)) in alternatively organized complexes with a (CG)6 DNA oligonucleotide at 2 and 1.8 Å resolution. These structures reveal novel aspects of the Zalpha interaction with DNA, and they give insights on the arrangement of multiple Zalpha domains on DNA helices longer than the minimal binding site.pt_PT
dc.description.sponsorshipFCT fellowship: (SFRH/BPD/71629/2010), ESRF BAG program, Instituto Gulbenkian de Ciência.pt_PT
dc.language.isoengpt_PT
dc.publisherOxford University Presspt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/112962/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/231000/EUpt_PT
dc.rightsopenAccesspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAmino Acid Sequencept_PT
dc.subjectDNA, Z-Formpt_PT
dc.subjectModels, Molecularpt_PT
dc.subjectMolecular Sequence Datapt_PT
dc.subjectProtein Kinasespt_PT
dc.subjectProtein Structure, Tertiarypt_PT
dc.subjectZebrafish Proteinspt_PT
dc.titleStructural basis for Z-DNA binding and stabilization by the zebrafish Z-DNA dependent protein kinase PKZpt_PT
dc.typearticlept_PT
degois.publication.firstPage9924pt_PT
degois.publication.issue21pt_PT
degois.publication.lastPage9933pt_PT
degois.publication.titleNucleic Acids Researchpt_PT
dc.relation.publisherversionhttp://nar.oxfordjournals.org/content/41/21/9924.longpt_PT
dc.peerreviewedyespt_PT
degois.publication.volume41pt_PT
dc.identifier.doi10.1093/nar/gkt743-
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