Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/494
Título: Molecular cytogenetic analysis of the crucian carp, Carassius carassius (Linnaeus, 1758) (Teleostei, Cyprinidae), using chromosome staining and fluorescence in situ hybridisation with rDNA probes
Autor: Spoz, Aneta
Boron, Alicja
Porycka, Katarzyna
Karolewska, Monika
Ito, Daisuke
Abe, Syuiti
Kirtiklis, Lech
Juchno, Dorota
Palavras-chave: Cyprinidae
CMA
FISH with rDNA
molecular cytogenetics
NOR-phenotype
polyploid species
Data: 18-Ago-2014
Editora: Pensosoft Publishers
Citação: Boron A, Spoz A, Porycka K, Karolewska M, Ito D, Abe S, Kirtiklis L, Juchno D (2014) Molecular cytogenetic analysis of the crucian carp, Carassius carassius (Linnaeus, 1758) (Teleostei, Cyprinidae), using chromosome staining and fluorescence in situ hybridisation with rDNA probes. Comparative Cytogenetics 8(3): 233-248. doi: 10.3897/compcytogen.v8i3.7718
Resumo: The crucian carp Carassius carassius (Linnaeus, 1758) is a species with restricted and decreasing distribution in Europe. Six males and six females of the species from the Baltic Sea basin in Poland were examined to show sequentially CMA3/AgNO3 staining pattern, DAPI staining, and, for the first time in literature, molecular cytogenetic analysis using double-colour fluorescence in situ hybridisation (FISH) with 28S and 5S rDNA probes. The karyotype consisted of 20 m, 36 sm and 44 sta chromosomes, NF=156. The AgNO3 stained NORs were most frequently located terminally in the short arms of two sm and two sta elements, and CMA3-positive sites were also observed suggesting abundant GC-rich repetitive DNA in the regions. Other CMA3-positive sites in the short arms of six to ten sm and sta chromosomes were detected. The results based on 28S rDNA FISH confirmed the location of rDNA sites. DAPI-negative staining of NORs suggested the scarcity of AT-rich DNA in the regions. FISH with 5S rDNA probe revealed 8-14 loci (ten and 12 in respectively 49 and 29% of metaphases). They were located in two sm and eight to ten sta chromosomes and six of them were larger than others. Simultaneously, mapping of the two rDNA families on the chromosomes of C. carassius revealed that both 28S and 5S rDNA probes were located in different chromosomes. Molecular cytogenetic data of C. carassius presented here for the first time give an important insight into the structure of chromosomes of this polyploid and declining species and may be useful in its systematics.
Peer review: yes
URI: http://hdl.handle.net/10400.7/494
DOI: 10.3897/CompCytogen.v8i3.7718
Versão do Editor: http://compcytogen.pensoft.net/articles.php?id=1815
Aparece nas colecções:CCR - Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
CompCytogen-008-233.pdfartigo principal2,06 MBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.