Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/510
Título: Independent recruitment of Igh alleles in V(D)J recombination
Autor: Alves-Pereira, Clara F.
de Freitas, Raquel
Lopes, Telma
Gardner, Rui
Marta, Filipa
Vieira, Paulo
Barreto, Vasco M.
Palavras-chave: Base Sequence
Cell Differentiation
Clone Cells
Epigenesis, Genetic
Gene Expression Regulation
Biological sciences
Immunology
V(D)J Recombination
Alleles
Data: 17-Dez-2014
Editora: Nature Publishing Group
Citação: Pereira, C. F. A. et al. Independent recruitment of Igh alleles in V(D)J recombination. Nat. Commun. 5:5623 doi: 10.1038/ncomms6623 (2014).
Resumo: How the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo V(H) to DJ(H) rearrangement. We also observe this similar probability in clones from multipotent and common lymphoid precursors. The extreme biases in the expression of the alleles that we find in more differentiated subsets are mostly due to constraints imposed by early rearrangements. Our data demonstrate that each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination.
Peer review: yes
URI: http://hdl.handle.net/10400.7/510
DOI: 10.1038/ncomms6623
Versão do Editor: http://www.nature.com/ncomms/2014/141217/ncomms6623/full/ncomms6623.html
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