Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/524
Título: Requirement for highly efficient pre-mRNA splicing duringDrosophilaearly embryonic development
Autor: Guilgur, Leonardo Gastón
Prudêncio, Pedro
Sobral, Daniel
Liszekova, Denisa
Rosa, André
Martinho, Rui Gonçalo
Palavras-chave: Prp19/NTC
early zygotic genes
fast development
mRNA splicing
Data: 22-Abr-2014
Editora: Elife Sciences Publications
Citação: eLife 2014;3:e02181
Resumo: Drosophila syncytial nuclear divisions limit transcription unit size of early zygotic genes. As mitosis inhibits not only transcription, but also pre-mRNA splicing, we reasoned that constraints on splicing were likely to exist in the early embryo, being splicing avoidance a possible explanation why most early zygotic genes are intronless. We isolated two mutant alleles for a subunit of the NTC/Prp19 complexes, which specifically impaired pre-mRNA splicing of early zygotic but not maternally encoded transcripts. We hypothesized that the requirements for pre-mRNA splicing efficiency were likely to vary during development. Ectopic maternal expression of an early zygotic pre-mRNA was sufficient to suppress its splicing defects in the mutant background. Furthermore, a small early zygotic transcript with multiple introns was poorly spliced in wild-type embryos. Our findings demonstrate for the first time the existence of a developmental pre-requisite for highly efficient splicing during Drosophila early embryonic development and suggest in highly proliferative tissues a need for coordination between cell cycle and gene architecture to ensure correct gene expression and avoid abnormally processed transcripts. DOI: http://dx.doi.org/10.7554/eLife.02181.001.
Peer review: yes
URI: http://hdl.handle.net/10400.7/524
DOI: 10.7554/eLife.02181
Versão do Editor: http://elifesciences.org/content/3/e02181
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