Utilize este identificador para referenciar este registo:
|Título:||The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation|
Olaseun, Victoria A.
|Palavras-chave:||HERC3 protein, human|
|Editora:||Oxford University Press|
|Citação:||Karin Hochrainer, Nadja Pejanovic, Victoria A. Olaseun, Sheng Zhang, Costantino Iadecola, and Josef Anrather The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation Nucl. Acids Res. (16 November 2015) 43 (20): 9889-9904 first published online October 17, 2015 doi:10.1093/nar/gkv1064|
|Resumo:||Activation of NF-κB-dependent transcription represents an important hallmark of inflammation. While the acute inflammatory response is per se beneficial, it can become deleterious if its spatial and temporal profile is not tightly controlled. Classically, NF-κB activity is limited by cytoplasmic retention of the NF-κB dimer through binding to inhibitory IκB proteins. However, increasing evidence suggests that NF-κB activity can also be efficiently contained by direct ubiquitination of NF-κB subunits. Here, we identify the HECT-domain ubiquitin ligase HERC3 as novel negative regulator of NF-κB activity. We find that HERC3 restricts NF-κB nuclear import and DNA binding without affecting IκBα degradation. Instead HERC3 indirectly binds to the NF-κB RelA subunit after liberation from IκBα inhibitor leading to its ubiquitination and protein destabilization. Remarkably, the regulation of RelA activity by HERC3 is independent of its inherent ubiquitin ligase activity. Rather, we show that HERC3 and RelA are part of a multi-protein complex containing the proteasome as well as the ubiquitin-like protein ubiquilin-1 (UBQLN1). We present evidence that HERC3 and UBQLN1 provide a link between NF-κB RelA and the 26S proteasome, thereby facilitating RelA protein degradation. Our findings establish HERC3 as novel candidate regulating the inflammatory response initiated by NF-κB.|
|Versão do Editor:||http://nar.oxfordjournals.org/content/43/20/9889.long|
|Aparece nas colecções:||I- Artigos|
Ficheiros deste registo:
|Nucl. Acids Res.-2015-Hochrainer-9889-904.pdf||artigo principal||9,61 MB||Adobe PDF||Ver/Abrir|
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.