Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/541
Título: Evolution of Escherichia coli to Macrophage Cell Line
Autor: Miskinyte, Migla
Gordo, Isabel
Palavras-chave: Microbiology
Microbe-host interactions
Microbial genetics
DNA replication
Molecular Biology
Escherichia coli
Data: Set-2014
Editora: Bio-Protocol
Citação: Miskinyte, M. and Gordo, I. (2014). Evolution of Escherichia coli to Macrophage Cell Line. Bio-protocol 4(17): e1227. http://www.bio-protocol.org/e1227
Resumo: The genomes of species of Escherichia coli (E. coli) show an extraordinary amount of diversity, which include commensal strains and strains belonging to different pathovars. Many strains of E. coli, which can cause mild or severe pathologies in humans, have a commensal ancestor. Understanding the evolutionary changes that can lead to a transition from commensal to pathogen is an important task, which requires integration of different methodologies. One method is experimental evolution of bacteria, in controlled environments, that mimic some of the selective pressures, likely to be important during the transition to pathogenesis. The success of such a transition will depend, at least partially, on ability of E. coli to adapt to the presence of cells of the immune system. Here, we describe a protocol for performing experimental evolution of a commensal strain of E. coli, a derivative of the well studied K12, under the constant selective pressure imposed by cells of the innate immune system, specifically RAW 264.7 murine macrophage cell line.
Peer review: yes
URI: http://hdl.handle.net/10400.7/541
Versão do Editor: http://www.bio-protocol.org/e1227
Aparece nas colecções:EB - Articles

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