Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/627
Título: Chromosomes. CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere
Autor: Falk, S. J.
Guo, L. Y.
Sekulic, N.
Smoak, E. M.
Mani, T.
Logsdon, G. A.
Gupta, K.
Jansen, L. E. T.
Van Duyne, G. D.
Vinogradov, S. A.
Lampson, M. A.
Black, B. E.
Palavras-chave: Autoantigens
Centromere
Chromosomal Proteins, Non-Histone
DNA
Epigenesis, Genetic
Fluorescence Resonance Energy Transfer
Gene Knockdown Techniques
Humans
Nucleosomes
Protein Structure, Secondary
Data: 8-Mai-2015
Editora: American Association for the Advancement of Science
Citação: CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere By Samantha J. Falk, Lucie Y. Guo, Nikolina Sekulic, Evan M. Smoak, Tomoyasu Mani, Glennis A. Logsdon, Kushol Gupta, Lars E. T. Jansen, Gregory D. Van Duyne, Sergei A. Vinogradov, Michael A. Lampson, Ben E. Black Science08 May 2015 : 699-703
Resumo: Inheritance of each chromosome depends upon its centromere. A histone H3 variant, centromere protein A (CENP-A), is essential for epigenetically marking centromere location. We find that CENP-A is quantitatively retained at the centromere upon which it is initially assembled. CENP-C binds to CENP-A nucleosomes and is a prime candidate to stabilize centromeric chromatin. Using purified components, we find that CENP-C reshapes the octameric histone core of CENP-A nucleosomes, rigidifies both surface and internal nucleosome structure, and modulates terminal DNA to match the loose wrap that is found on native CENP-A nucleosomes at functional human centromeres. Thus, CENP-C affects nucleosome shape and dynamics in a manner analogous to allosteric regulation of enzymes. CENP-C depletion leads to rapid removal of CENP-A from centromeres, indicating their collaboration in maintaining centromere identity.
Peer review: yes
URI: http://hdl.handle.net/10400.7/627
DOI: 10.1126/science.1259308
Versão do Editor: http://science.sciencemag.org/content/348/6235/699.long
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