Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/747
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dc.contributor.authorDeaton, Aimee M-
dc.contributor.authorGómez-Rodríguez, Mariluz-
dc.contributor.authorMieczkowski, Jakub-
dc.contributor.authorTolstorukov, Michael Y-
dc.contributor.authorKundu, Sharmistha-
dc.contributor.authorSadreyev, Ruslan I-
dc.contributor.authorJansen, Lars ET-
dc.contributor.authorKingston, Robert E-
dc.date.accessioned2017-04-10T17:22:05Z-
dc.date.available2017-04-10T17:22:05Z-
dc.date.issued2016-06-15-
dc.identifier.citationeLife 2016;5:e15316pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/747-
dc.descriptionData availability - High throughput sequencing data has been deposited in GEO and is accessible using the following links: Time-ChIP: GSE78876 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE78876 ChIP-seq: GSE78899 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE78899 MNase titration and RNA-seq: GSE78984 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE78984pt_PT
dc.description.abstractThe organization of DNA into chromatin is dynamic; nucleosomes are frequently displaced to facilitate the ability of regulatory proteins to access specific DNA elements. To gain insight into nucleosome dynamics, and to follow how dynamics change during differentiation, we used a technique called time-ChIP to quantitatively assess histone H3.3 turnover genome-wide during differentiation of mouse ESCs. We found that, without prior assumptions, high turnover could be used to identify regions involved in gene regulation. High turnover was seen at enhancers, as observed previously, with particularly high turnover at super-enhancers. In contrast, regions associated with the repressive Polycomb-Group showed low turnover in ESCs. Turnover correlated with DNA accessibility. Upon differentiation, numerous changes in H3.3 turnover rates were observed, the majority of which occurred at enhancers. Thus, time-ChIP measurement of histone turnover shows that active enhancers are unusually dynamic in ESCs and changes in highly dynamic nucleosomes predominate at enhancers during differentiation.pt_PT
dc.description.sponsorshipNational Institutes of Health grant: (NIH R01 GM48405); European Molecular Biology Organization grant: (installation grant 1818); European Research Council.pt_PT
dc.language.isoengpt_PT
dc.publishereLife Sciences Publicationspt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/100537/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F33567%2F2008/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/615638/EUpt_PT
dc.rightsopenAccesspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectgenes and chromosomespt_PT
dc.subjectchromatinpt_PT
dc.subjectdifferentiationpt_PT
dc.subjecthistone H3.3pt_PT
dc.subjectMousept_PT
dc.subjectstem cellspt_PT
dc.subjectturnoverpt_PT
dc.titleEnhancer regions show high histone H3.3 turnover that changes during differentiationpt_PT
dc.typearticlept_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
degois.publication.firstPage1pt_PT
degois.publication.lastPage24pt_PT
degois.publication.titleeLifept_PT
dc.relation.publisherversionhttps://elifesciences.org/content/5/e15316pt_PT
dc.peerreviewedyespt_PT
degois.publication.volume5pt_PT
dc.identifier.doi10.7554/eLife.15316pt_PT
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