Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/752
Título:  -Tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells
Autor: Kuriyama, R.
Bettencourt-Dias, M.
Hoffmann, I.
Arnold, M.
Sandvig, L.
Palavras-chave: Centrioles
Colorectal Neoplasms
Humans
Hydroxyurea
Microtubules
Nocodazole
Protein-Serine-Threonine Kinases
Spindle Apparatus
Tubulin
Tubulin Modulators
Up-Regulation
HCT116 Cells
Data: 3-Jun-2009
Editora: Company of Biologists
Citação: Gamma -Tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells Ryoko Kuriyama, Monica Bettencourt-Dias, Ingrid Hoffmann, Marc Arnold, Lisa Sandvig J Cell Sci 2009 122: 2014-2023; doi: 10.1242/jcs.036715
Resumo: Cancer cells frequently induce aberrant centrosomes, which have been implicated in cancer initiation and progression. Human colorectal cancer cells, HCT116, contain aberrant centrioles composed of disorganized cylindrical microtubules and displaced appendages. These cells also express unique centrosome-related structures associated with a subset of centrosomal components, including gamma-tubulin, centrin and PCM1. During hydroxyurea treatment, these abnormal structures become more abundant and undergo a change in shape from small dots to elongated fibers. Although gamma-tubulin seems to exist as a ring complex, the abnormal structures do not support microtubule nucleation. Several lines of evidence suggest that the fibers correspond to a disorganized form of centriolar microtubules. Plk4, a mammalian homolog of ZYG-1 essential for initiation of centriole biogenesis, is not associated with the gamma-tubulin-specific abnormal centrosomes. The amount of Plk4 at each centrosome was less in cells with abnormal centrosomes than cells without gamma-tubulin-specific abnormal centrosomes. In addition, the formation of abnormal structures was abolished by expression of exogenous Plk4, but not SAS6 and Cep135/Bld10p, which are downstream regulators required for the organization of nine-triplet microtubules. These results suggest that HCT116 cells fail to organize the ninefold symmetry of centrioles due to insufficient Plk4.
Descrição: The article hasn't a creative commons license associated nor informations about funders/sponsors. There is no public supplementary material available.
Peer review: yes
URI: http://hdl.handle.net/10400.7/752
DOI: 10.1242/jcs.036715
Versão do Editor: http://jcs.biologists.org/content/122/12/2014
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