Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/756
Título: Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells
Autor: Tavares, Sandra
Vieira, André Filipe
Taubenberger, Anna Verena
Araújo, Margarida
Martins, Nuno Pimpao
Brás-Pereira, Catarina
Polónia, António
Herbig, Maik
Barreto, Clara
Otto, Oliver
Cardoso, Joana
Pereira-Leal, José B.
Guck, Jochen
Paredes, Joana
Janody, Florence
Palavras-chave: Biophysics
Data: 16-Mai-2017
Editora: Nature Publishing Group
Citação: Tavares, S. et al. Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells. Nat. Commun. 8, 15237 doi: 10.1038/ncomms15237 (2017).
Resumo: Studies of the role of actin in tumour progression have highlighted its key contribution in cell softening associated with cell invasion. Here, using a human breast cell line with conditional Src induction, we demonstrate that cells undergo a stiffening state prior to acquiring malignant features. This state is characterized by the transient accumulation of stress fibres and upregulation of Ena/VASP-like (EVL). EVL, in turn, organizes stress fibres leading to transient cell stiffening, ERK-dependent cell proliferation, as well as enhancement of Src activation and progression towards a fully transformed state. Accordingly, EVL accumulates predominantly in premalignant breast lesions and is required for Src-induced epithelial overgrowth in Drosophila. While cell softening allows for cancer cell invasion, our work reveals that stress fibre-mediated cell stiffening could drive tumour growth during premalignant stages. A careful consideration of the mechanical properties of tumour cells could therefore offer new avenues of exploration when designing cancer-targeting therapies.
Descrição: This deposit is composed by the main article and supplementary files of the publication.
Peer review: yes
URI: http://hdl.handle.net/10400.7/756
DOI: 10.1038/ncomms15237
Versão do Editor: https://www.nature.com/articles/ncomms15237
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Tavares_ncomms.8-15237.pdfmain article6,13 MBAdobe PDFVer/Abrir
Tavares_ncomms.8-15237-s1.pdfsupplementary material 15,57 MBAdobe PDFVer/Abrir
Tavares_ncomms.8-15237- Peer Review File.pdfPeer Review file544,58 kBAdobe PDFVer/Abrir
Tavares_ncomms.8-15237-s2.xlssupplementary material 21,85 MBMicrosoft ExcelVer/Abrir
Tavares_ncomms.8-15237-s3.xlsxsupplementary material 327,48 kBMicrosoft Excel XMLVer/Abrir
Tavares_ncomms.8-15237-s4.movsupplementary material 44,24 MBVideo QuicktimeVer/Abrir
Tavares_ncomms.8-15237-s5.movsupplementary material 57,25 MBVideo QuicktimeVer/Abrir
Tavares_ncomms.8-15237-s6.movsupplementary material 62,08 MBVideo QuicktimeVer/Abrir
Tavares_ncomms.8-15237-s7.movsupplementary material 72,18 MBVideo QuicktimeVer/Abrir

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