Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/775
Título: Heterologous expression of the yeast Tpo1p or Pdr5p membrane transporters in Arabidopsis confers plant xenobiotic tolerance
Autor: Remy, Estelle
Niño-González, María
Godinho, Cláudia P.
Cabrito, Tânia R.
Teixeira, Miguel C.
Sá-Correia, Isabel
Duque, Paula
Palavras-chave: Abiotic
Molecular engineering in plants
Data: 3-Jul-2017
Editora: Nature Publishing Group
Citação: Remy, E. et al. Heterologous expression of the yeast Tpo1p or Pdr5p membrane transporters in Arabidopsis confers plant xenobiotic tolerance. Sci Rep 7, 4529 (2017). doi:10.1038/s41598-017-04534-7
Resumo: Soil contamination is a major hindrance for plant growth and development. The lack of effective strategies to remove chemicals released into the environment has raised the need to increase plant resilience to soil pollutants. Here, we investigated the ability of two Saccharomyces cerevisiae plasma-membrane transporters, the Major Facilitator Superfamily (MFS) member Tpo1p and the ATP-Binding Cassette (ABC) protein Pdr5p, to confer Multiple Drug Resistance (MDR) in Arabidopsis thaliana. Transgenic plants expressing either of the yeast transporters were undistinguishable from the wild type under control conditions, but displayed tolerance when challenged with the herbicides 2,4-D and barban. Plants expressing ScTPO1 were also more resistant to the herbicides alachlor and metolachlor as well as to the fungicide mancozeb and the Co(2+), Cu(2+), Ni(2+), Al(3+) and Cd(2+) cations, while ScPDR5-expressing plants exhibited tolerance to cycloheximide. Yeast mutants lacking Tpo1p or Pdr5p showed increased sensitivity to most of the agents tested in plants. Our results demonstrate that the S. cerevisiae Tpo1p and Pdr5p transporters are able to mediate resistance to a broad range of compounds of agricultural interest in yeast as well as in Arabidopsis, underscoring their potential in future biotechnological applications.
Descrição: This deposit is composed by the main article plus the supplementary materials of the publication.
Peer review: yes
URI: http://hdl.handle.net/10400.7/775
DOI: 10.1038/s41598-017-04534-7
Versão do Editor: https://www.nature.com/articles/s41598-017-04534-7
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Duque_SciRep2017_SM.pdfsupplementary material1,51 MBAdobe PDFVer/Abrir


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