Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/832
Título: Methods to Study Centrosomes and Cilia in Drosophila
Autor: Jana, Swadhin Chandra
Mendonça, Susana
Werner, Sascha
Bettencourt-Dias, Monica
Palavras-chave: Drosophila
Basal body
Intraflagellar transport
Sensory neuron
Data: 12-Ago-2016
Editora: Humana Press
Citação: Jana S.C., Mendonça S., Werner S., Bettencourt-Dias M. (2016) Methods to Study Centrosomes and Cilia in Drosophila. In: Satir P., Christensen S. (eds) Cilia. Methods in Molecular Biology, vol 1454. Humana Press, New York, NY
Resumo: Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.
Descrição: The deposited item is a book chapter and is part of the series " Methods in Molecular Biology book series ([MIMB, volume 1454]) published by the publisher Humana Press.
The deposited book chapter is a pre-print version and hasn't been submitted to peer reviewing.
There is no public supplementary material available for this publication.
This publication hasn't any creative commons license associated.
Peer review: no
URI: http://hdl.handle.net/10400.7/832
DOI: 10.1007/978-1-4939-3789-9_14
Versão do Editor: https://link.springer.com/protocol/10.1007%2F978-1-4939-3789-9_14
Aparece nas colecções:CCR - Book Chapters

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