Please use this identifier to cite or link to this item:
Title: 1,25-Dihydroxyvitamin D3 promotes tolerogenic dendritic cells with functional migratory properties in NOD mice
Author: Ferreira, Gabriela B
Gysemans, Conny A
Demengeot, Jocelyne
da Cunha, João Paulo M C M
Vanherwegen, An-Sofie
Overbergh, Lut
Van Belle, Tom L
Pauwels, Femke
Verstuyf, Annemieke
Korf, Hannelie
Mathieu, Chantal
Keywords: Animals
Chemotaxis, Leukocyte
Dendritic Cells
Flow Cytometry
Fluorescent Antibody Technique
Immune Tolerance
Lymphocyte Activation
Mice, Inbred C57BL
Mice, Inbred NOD
Mice, Transgenic
Real-Time Polymerase Chain Reaction
Vitamin D
Issue Date: 1-May-2014
Publisher: American Association of Immunologists
Citation: 1,25-Dihydroxyvitamin D3 Promotes Tolerogenic Dendritic Cells with Functional Migratory Properties in NOD Mice Gabriela B. Ferreira, Conny A. Gysemans, Jocelyne Demengeot, João Paulo M. C. M. da Cunha, An-Sofie Vanherwegen, Lut Overbergh, Tom L. Van Belle, Femke Pauwels, Annemieke Verstuyf, Hannelie Korf, Chantal Mathieu The Journal of Immunology May 1, 2014, 192 (9) 4210-4220; DOI: 10.4049/jimmunol.1302350
Abstract: The biologically active form of vitamin D, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], is able to promote the generation of tolerogenic mature dendritic cells (mDCs) with an impaired ability to activate autoreactive T cells. These cells could represent a reliable tool for the promotion or restoration of Ag-specific tolerance through vaccination strategies, for example in type 1 diabetes patients. However, successful transfer of 1,25(OH)2D3-treated mDCs (1,25D3-mDCs) depends on the capacity of 1,25(OH)2D3 to imprint a similar tolerogenic profile in cells derived from diabetes-prone donors as from diabetes-resistant donors. In this study, we examined the impact of 1,25(OH)2D3 on the function and phenotype of mDCs originating from healthy (C57BL/6) and diabetes-prone (NOD) mice. We show that 1,25(OH)2D3 is able to imprint a phenotypic tolerogenic profile on DCs derived from both mouse strains. Both NOD- and C57BL/6-derived 1,25D3-mDCs decreased the proliferation and activation of autoreactive T cells in vitro, despite strain differences in the regulation of cytokine/chemokine expression. In addition, 1,25D3-mDCs from diabetes-prone mice expanded CD25(+)Foxp3(+) regulatory T cells and induced intracellular IL-10 production by T cells in vitro. Furthermore, 1,25D3-mDCs exhibited an intact functional migratory capacity in vivo that favors homing to the liver and pancreas of adult NOD mice. More importantly, when cotransferred with activated CD4(+) T cells into NOD.SCID recipients, 1,25D3-mDCs potently dampened the proliferation of autoreactive donor T cells in the pancreatic draining lymph nodes. Altogether, these results argue for the potential of 1,25D3-mDCs to restore Ag-specific immune tolerance and arrest autoimmune disease progression in vivo.
Description: This deposit is composed by a publication in which the IGC's authors have had the role of collaboration (it's a collaboration publication). This type of deposit in ARCA is in restrictedAccess (it can't be in open access to the public), and can only be accessed by two ways: either by requesting a legal copy from the author (the email contact present in this deposit) or by visiting the following link:
This publication hasn't any creative commons license associated.
Peer review: yes
DOI: 10.4049/jimmunol.1302350
Publisher Version:
Appears in Collections:LP - Artigos

Files in This Item:
File Description SizeFormat 
Mathieu_J.Immunol._(2014).pdfmain article1,71 MBAdobe PDFView/Open    Request a copy

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.