Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.7/865
Title: Assessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlings
Author: Remy, Estelle
Duque, Paula
Keywords: Arabidopsis thaliana
Chlorophyll content
Heavy-metal stress tolerance
Lateral root development
Primary root elongation
Root assay
Seedlings
Shoot biomass
Issue Date: Feb-2016
Publisher: Springer Publishing Company
Citation: Remy,E., Duque, P. (2016). Methods Mol.Biol. 1398:197-208. doi: 10.1007/978-1-4939-3356-3_16.
Abstract: Heavy-metal soil contamination is one of the major abiotic stress factors that, by negatively affecting plant growth and development, severely limit agricultural productivity worldwide. Plants have evolved various tolerance and detoxification strategies in order to cope with heavy-metal toxicity while ensuring adequate supply of essential micronutrients at the whole-plant as well as cellular levels. Genetic studies in the model plant Arabidopsis thaliana have been instrumental in elucidating such mechanisms. The root assay constitutes a very powerful and simple method to assess heavy-metal stress tolerance in Arabidopsis seedlings. It allows the simultaneous determination of all the standard growth parameters affected by heavy-metal stress (primary root elongation, lateral root development, shoot biomass, and chlorophyll content) in a single experiment. Additionally, this protocol emphasizes the tips and tricks that become particularly useful when quantifying subtle alterations in tolerance to a given heavy-metal stress, when simultaneously pursuing a large number of plant lines, or when testing sensitivity to a wide range of heavy metals for a single line.
Description: The deposited book chapter is a post-print version and has been submitted to peer review.
The deposited book chapter version contains attached the supplementary materials within the pdf.
This publication hasn't any creative commons license associated.
The deposited book chapter is part of the book series: "Environmental Responses in Plants: Methods and Protocols" (pp.197-208) published by Springer.
Peer review: yes
URI: http://hdl.handle.net/10400.7/865
DOI: 10.1007/978-1-4939-3356-3_16
ISSN: 1064-3745
1940-6029
Publisher Version: https://journals.cshl.edu:2443/login?qurl=https%3a%2f%2flink.springer.com%2f10.1007%2f978-1-4939-3356-3_16
Appears in Collections:PMB - Book Chapters

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