Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.7/866
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dc.contributor.authorSemedo-Aguiar, Ana-
dc.contributor.authorPereira-Leal, Jose-
dc.contributor.authorLeite, Ricardo-
dc.date.accessioned2018-05-15T11:23:53Z-
dc.date.available2018-05-15T11:23:53Z-
dc.date.issued2018-05-05-
dc.identifier.citationSemedo-Aguiar, A.P.; Pereira-Leal, J.B.; Leite, R.B. Microbial Diversity and Toxin Risk in Tropical Freshwater Reservoirs of Cape Verde. Toxins 2018, 10, 186.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/866-
dc.descriptionThis deposit is composed by the main article plus the supplementary materials of the publication.pt_PT
dc.description.abstractThe Cape Verde islands are part of the African Sahelian arid belt that possesses an erratic rain pattern prompting the need for water reservoirs, which are now critical for the country’s sustainability. Worldwide, freshwater cyanobacterial blooms are increasing in frequency due to global climate change and the eutrophication of water bodies, particularly in reservoirs. To date, there have been no risk assessments of cyanobacterial toxin production in these man-made structures. We evaluated this potential risk using 16S rRNA gene amplicon sequencing and full metagenome sequencing in freshwater reservoirs of Cape Verde. Our analysis revealed the presence of several potentially toxic cyanobacterial genera in all sampled reservoirs. Faveta potentially toxic and bloom-forming Microcystis sp., dominated our samples, while a Cryptomonas green algae and Gammaproteobacteria dominated Saquinho and Poilão reservoirs. We reconstructed and assembled the Microcystis genome, extracted from the metagenome of bulk DNA from Faveta water. Phylogenetic analysis of Microcystis cf. aeruginosa CV01’s genome revealed its close relationship with other Microcystis genomes, as well as clustering with other continental African strains, suggesting geographical coherency. In addition, it revealed several clusters of known toxin-producing genes. This survey reinforces the need to better understand the country’s microbial ecology as a whole of water reservoirs on the rise.pt_PT
dc.description.sponsorshipPortuguese Science and Technology Foundation grants: (PTDC/MAR-BIO/4132/2014, SFRH/BD/113752/2015, SFRH/BPD/91518/2012).pt_PT
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.relationPTDC/MAR-BIO/4132/2014pt_PT
dc.relationSFRH/BD/113752/2015pt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F91518%2F2012/PTpt_PT
dc.rightsopenAccesspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjecttoxinspt_PT
dc.subjectCyanobacteriapt_PT
dc.subjectNGSpt_PT
dc.subjectMicrocystispt_PT
dc.subjectMAGspt_PT
dc.subjectmetagenomept_PT
dc.subjectfreshwaterpt_PT
dc.subjectmicrobial communitiespt_PT
dc.subjectCape Verdept_PT
dc.subjectreservoirspt_PT
dc.titleMicrobial Diversity and Toxin Risk in Tropical Freshwater Reservoirs of Cape Verdept_PT
dc.typearticlept_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
degois.publication.firstPage1pt_PT
degois.publication.issue5pt_PT
degois.publication.lastPage19pt_PT
degois.publication.titleToxinspt_PT
dc.relation.publisherversionhttp://www.mdpi.com/2072-6651/10/5/186pt_PT
dc.peerreviewedyespt_PT
degois.publication.volume10pt_PT
dc.identifier.doi10.3390/toxins10050186pt_PT
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Leite,R._Toxins_(2018).pdfmain article6,91 MBAdobe PDFVer/Abrir
Leite,R._Toxins_(2018)_SM_.pdfsupplementary materials458,91 kBAdobe PDFVer/Abrir


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