Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.7/868
Title: Ferritin H Deficiency in Myeloid Compartments Dysregulates Host Energy Metabolism and Increases Susceptibility to Mycobacterium tuberculosis Infection
Author: Reddy, Vineel P.
Chinta, Krishna C.
Saini, Vikram
Glasgow, Joel N.
Hull, Travis D.
Traylor, Amie
Rey-Stolle, Fernanda
Soares, Miguel P.
Madansein, Rajhmun
Rahman, Md Aejazur
Barbas, Coral
Nargan, Kievershen
Naidoo, Threnesan
Ramdial, Pratistadevi K.
George, James F.
Agarwal, Anupam
Steyn, Adrie J. C.
Keywords: ferritin H chain
bioenergetics
immunometabolism
tuberculosis
iron
energy metabolism
Issue Date: 3-May-2018
Publisher: Frontiers Media
Citation: Reddy VP, Chinta KC, Saini V, Glasgow JN, Hull TD, Traylor A, Rey-Stolle F, Soares MP, Madansein R, Rahman MA, Barbas C, Nargan K, Naidoo T, Ramdial PK, George JF, Agarwal A and Steyn AJC (2018) Ferritin H Deficiency in Myeloid Compartments Dysregulates Host Energy Metabolism and Increases Susceptibility to Mycobacterium tuberculosis Infection. Front. Immunol. 9:860. doi: 10.3389/fimmu.2018.00860
Abstract: Iron is an essential factor for the growth and virulence of Mycobacterium tuberculosis (Mtb). However, little is known about the mechanisms by which the host controls iron availability during infection. Since ferritin heavy chain (FtH) is a major intracellular source of reserve iron in the host, we hypothesized that the lack of FtH would cause dysregulated iron homeostasis to exacerbate TB disease. Therefore, we used knockout mice lacking FtH in myeloid-derived cell populations to study Mtb disease progression. We found that FtH plays a critical role in protecting mice against Mtb, as evidenced by increased organ burden, extrapulmonary dissemination, and decreased survival in Fth−/− mice. Flow cytometry analysis showed that reduced levels of FtH contribute to an excessive inflammatory response to exacerbate disease. Extracellular flux analysis showed that FtH is essential for maintaining bioenergetic homeostasis through oxidative phosphorylation. In support of these findings, RNAseq and mass spectrometry analyses demonstrated an essential role for FtH in mitochondrial function and maintenance of central intermediary metabolism in vivo. Further, we show that FtH deficiency leads to iron dysregulation through the hepcidin–ferroportin axis during infection. To assess the clinical significance of our animal studies, we performed a clinicopathological analysis of iron distribution within human TB lung tissue and showed that Mtb severely disrupts iron homeostasis in distinct microanatomic locations of the human lung. We identified hemorrhage as a major source of metabolically inert iron deposition. Importantly, we observed increased iron levels in human TB lung tissue compared to healthy tissue. Overall, these findings advance our understanding of the link between iron-dependent energy metabolism and immunity and provide new insight into iron distribution within the spectrum of human pulmonary TB. These metabolic mechanisms could serve as the foundation for novel host-directed strategies.
Description: This deposit is composed by the main article plus the supplementary materials of the publication.
This deposit is composed by a publication in which the IGC's authors have had the role of collaboration (it's a collaboration publication). This type of deposit in ARCA is in restrictedAccess (it can't be in open access to the public), and can only be accessed by two ways: either by requesting a legal copy from the author (the email contact present in this deposit) or by visiting the following link: https://www.frontiersin.org/articles/10.3389/fimmu.2018.00860/full
Peer review: yes
URI: http://hdl.handle.net/10400.7/868
DOI: 10.3389/fimmu.2018.00860
Publisher Version: https://www.frontiersin.org/articles/10.3389/fimmu.2018.00860/full
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