Rodrigues-Martins, ARiparbelli, MCallaini, GGlover, D MBettencourt-Dias, M2020-04-282020-04-282007-05-18http://hdl.handle.net/10400.7/955Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.engAnimalsAnimals, Genetically ModifiedCentriolesCentrosomeDrosophilaDrosophila ProteinsEmbryo, NonmammalianEmbryonic DevelopmentFemaleMitosisOocytesjournal article10.1126/science.1142950