Browsing by Author "Deshpande, P."
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- IgG autoantibody to brain beta tubulin III associated with cytokine cluster-II discriminate cerebral malaria in central IndiaPublication . Bansal, D.; Herbert, F.; Lim, P.; Deshpande, P.; Becavin, C.; Guiyedi, V.; de Maria, I.; Rousselle, J.C.; Namane, A.; Jain, R.; Cazenave, P.A.; Mishra, G.C.; Ferlini, C.; Fesel, C.; Benecke, A.; Pied, S.We investigated the significance of these self-reactive antibodies in clinically well-defined groups of P. falciparum infected patients manifesting mild malaria (MM), severe non-cerebral malaria (SM), or cerebral malaria (CM) and in control subjects from Gondia, a malaria epidemic site in central India using quantitative immunoprinting and multivariate statistical analyses. A two-fold complete-linkage hierarchical clustering allows classifying the different patient groups and to distinguish the CM from the others on the basis of their profile of IgG reactivity to brain proteins defined by PANAMA Blot. We identified beta tubulin III (TBB3) as a novel discriminant brain antigen in the prevalence of CM. In addition, circulating IgG from CM patients highly react with recombinant TBB3. Overall, correspondence analyses based on singular value decomposition show a strong correlation between IgG anti-TBB3 and elevated concentration of cluster-II cytokine (IFNγ, IL1β, TNFα, TGFβ) previously demonstrated to be a predictor of CM in the same population
- Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical statusPublication . Duarte, J.; Deshpande, P.; Guiyedi, V.; Mecheri, S.; Fesel, C.; Cazenave, P.A.; Mishra, G.C.; Kombila, M.; Pied, S.Blood samples were collected from controls and P. falciparum-infected patients before treatment on the day of hospitalization (day 0) in India and, in addition, on days 7 and 30 after treatment in Gabon. Total IgE levels were determined by ELISA and functional P. falciparum-specific IgE were estimated using a mast cell line RBL-2H3 transfected with a human Fcε RI α-chain that triggers degranulation upon human IgE cross-linking. Mann Whitney and Kruskall Wallis tests were used to compare groups and the Spearman test was used for correlations. Total IgE levels were confirmed to increase upon infection and differ with level of transmission and age but were not directly related to the disease phenotype. All studied groups exhibited functional parasite-specific IgEs able to induce mast cell degranulation in vitro in the presence of P. falciparum antigens. Plasma IgE levels correlated with those of IL-10 in uncomplicated malaria patients from Gabon. In Indian patients, plasma IFN-γ , TNF and IL-10 levels were significantly correlated with IgE concentrations in all groups.