Browsing by Author "Marques, C.C."
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- Cyclooxygenase-2 (COX-2) expression by in vitro produced bovine embryos. Preliminary results = Expressão da ciclo-oxigenase-2 (COX-2) em embriões bovinos produzidos in vitro. Resultados preliminaresPublication . Marques, C.C.; Horta, A.E.; Vasques, M.I.; Baptista, M.C.; Becker, J.D.; Pimenta, J.; Pereira, R.M.Expression of the two isoforms of cyclooxygenase enzyme, cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), by in vitro produced bovine embryos was examined. Cumulus-oocyte complexes were recovered from ovaries of slaughtered animals and subsequently in vitro matured and in vitro fertilized. Presumptive zygotes were cultured in serum containing medium (TCM 199+10% bovine superovulated oestrus serum) on a granulosa cell monolayer for 12 days.