Cytoplasmic sensing of Toxoplasma gondii infection and host cell programmed necrosis

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Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells

Publication . Alvarez, Catalina; Campos, Ana Claudia; Howard, Jonathan C; Loureiro, Joana; Müller, Urs Benedikt; Rodrigues, Ana Lina

Critical steps in resistance of mice against Toxoplasma gondii occur in the first 2 or 3 h after the pathogen has entered a cell that has been exposed to interferon γ (IFNγ). The newly formed parasitophorous vacuole is attacked by the IFNγ-inducible IRG proteins and disrupted, resulting in death of the parasite and necrotic death of the cell. Here we describe some techniques that we have used to describe and quantify these events in different combinations of the host and the parasite.

2020Capítulo de livro

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Entidade financiadora

European Commission

Programa de financiamento

H2020

Número da atribuição

708694