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Rab27a and Rab27b control different steps of the exosome secretion pathway

2010-01Journal article Open access
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dc.contributor.authorOstrowski, M.
dc.contributor.authorCarmo, NB.
dc.contributor.authorKrumeich, S.
dc.contributor.authorFanget, I.
dc.contributor.authorRaposo, G.
dc.contributor.authorSavina, A.
dc.contributor.authorMoita, CF.
dc.contributor.authorSchauer, K.
dc.contributor.authorHume, AN.
dc.contributor.authorFreitas, RP.
dc.contributor.authorGoud, B.
dc.contributor.authorBenaroch, P.
dc.contributor.authorHachoen, N.
dc.contributor.authorFukuda, M.
dc.contributor.authorDesnos, C.
dc.contributor.authorSeabra, MC.
dc.contributor.authorDarchen, F.
dc.contributor.authorAmigorena, S.
dc.contributor.authorMoita, LF.
dc.contributor.authorThery, C.
dc.date.accessioned2010-09-07T10:28:44Z
dc.date.available2010-09-07T10:28:44Z
dc.date.issued2010-01
dc.description.abstractExosomes are secreted membrane vesicles that share structural and biochemical characteristics with intraluminal vesicles of multivesicular endosomes (MVEs). Exosomes could be involved in intercellular communication and in the pathogenesis of infectious and degenerative diseases. The molecular mechanisms of exosome biogenesis and secretion are, however, poorly understood. Using an RNA interference (RNAi) screen, we identified five Rab GTPases that promote exosome secretion in HeLa cells. Among these, Rab27a and Rab27b were found to function in MVE docking at the plasma membrane. The size of MVEs was strongly increased by Rab27a silencing, whereas MVEs were redistributed towards the perinuclear region upon Rab27b silencing. Thus, the two Rab27 isoforms have different roles in the exosomal pathway. In addition, silencing two known Rab27 effectors, Slp4 (also known as SYTL4, synaptotagmin-like 4) and Slac2b (also known as EXPH5, exophilin 5), inhibited exosome secretion and phenocopied silencing of Rab27a and Rab27b, respectively. Our results therefore strengthen the link between MVEs and exosomes, and introduce ways of manipulating exosome secretion in vivo.por
dc.identifier.citationOstrowski M., Carmo NB., Krumeich S., Fanget I., Raposo G (Raposo, Graca)3,4, Savina A (Savina, Ariel)2,3, Moita CF., Schauer K., Hume AN., Freitas RP., Goud B., Benaroch P., Hacohen N., Fukuda M., Desnos, Claire, Seabra, Miguel C., Darchen F., Amigorena S., Moita LF., Thery C. (2010). “Rab27a and Rab27b control different steps of the exosome secretion pathway” Nature Cell Biology. 12 (1): 19-U61por
dc.identifier.issn1465-7392
dc.identifier.urihttp://hdl.handle.net/10400.7/200
dc.language.isoengpt
dc.publisherNaturepor
dc.subjectCELL-DERIVED EXOSOMESpor
dc.subjectMULTIVESICULAR ENDOSOMESpor
dc.subjectPLASMA-MEMBRANEpor
dc.subjectB-LYMPHOCYTESpor
dc.subjectVESICLESpor
dc.subjectPROTEINSpor
dc.subjectGRANULESpor
dc.subjectRELEASEpor
dc.subjectGTPASESpor
dc.subjectASSOCIATIONpor
dc.titleRab27a and Rab27b control different steps of the exosome secretion pathwaypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPageU61por
oaire.citation.startPage19por
oaire.citation.titleNature Cell Biologypor
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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