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Clustering of Rab11 vesicles in influenza A virus infected cells creates hotspots containing the 8 viral ribonucleoproteins

2016-05-26Journal article Open access
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dc.contributor.authorVale-Costa, Sílvia
dc.contributor.authorAmorim, Maria João
dc.date.accessioned2016-11-30T16:00:22Z
dc.date.available2016-11-30T16:00:22Z
dc.date.issued2016-05-26
dc.description.abstractInfluenza A virus is an important human pathogen causative of yearly epidemics and occasional pandemics. The ability to replicate within the host cell is a determinant of virulence, amplifying viral numbers for host-to-host transmission. This process requires multiple rounds of entering permissive cells, replication, and virion assembly at the plasma membrane, the site of viral budding and release. The assembly of influenza A virus involves packaging of several viral (and host) proteins and of a segmented genome, composed of 8 distinct RNAs in the form of viral ribonucleoproteins (vRNPs). The selective assembly of the 8-segment core remains one of the most interesting unresolved problems in virology. The recycling endosome regulatory GTPase Rab11 was shown to contribute to the process, by transporting vRNPs to the periphery, giving rise to enlarged cytosolic puncta rich in Rab11 and the 8 vRNPs. We recently reported that vRNP hotspots were formed of clustered vesicles harbouring protruding electron-dense structures that resembled vRNPs. Mechanistically, vRNP hotspots were formed as vRNPs outcompeted the cognate effectors of Rab11, the Rab11-Family-Interacting-Proteins (FIPs) for binding, and as a consequence impair recycling sorting at an unknown step. Here, we speculate on the impact that such impairment might have in host immunity, membrane architecture and viral assembly.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia grant: (IF/00899/2013).pt_PT
dc.identifier.citationSílvia Vale-Costa & Maria Jo ão Amorim (2016): Clustering of Rab11 vesicles in influenza A virus infected cells creates hotspots containing the 8 viral ribonucleoproteins, Small GTPases, DOI: 10.1080/21541248.2016.1199190pt_PT
dc.identifier.doi10.1080/21541248.2016.1199190pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/724
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relationMolecular characterization of the cellular machinery involved in genome trafficking of influenza A virus
dc.relation.publisherversionhttp://www.tandfonline.com/doi/full/10.1080/21541248.2016.1199190pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectCorrelative light and electron microscopypt_PT
dc.subjectinfluenza A virus assemblypt_PT
dc.subjectRab11 GTPasept_PT
dc.subjectRab11 family interacting proteins (FIPs)pt_PT
dc.subjectrecycling endosomept_PT
dc.subjectsegmented genomept_PT
dc.subjectviral ribonucleoproteins (vRNPs)pt_PT
dc.titleClustering of Rab11 vesicles in influenza A virus infected cells creates hotspots containing the 8 viral ribonucleoproteinspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular characterization of the cellular machinery involved in genome trafficking of influenza A virus
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F94204%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FIMI-MIC%2F1142%2F2012/PT
oaire.citation.endPage7pt_PT
oaire.citation.issue0pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleSmall GTPasespt_PT
oaire.citation.volume0pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationa6608970-1624-4621-a0df-f1c0cd22766f
relation.isProjectOfPublicationb3129c8c-ee05-4456-a36c-13de51208da7
relation.isProjectOfPublication.latestForDiscoveryb3129c8c-ee05-4456-a36c-13de51208da7

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