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Juvenile hormone regulates body size and perturbs insulin signaling in Drosophila

2014Journal article Open access
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dc.contributor.authorMirth, C. K.
dc.contributor.authorTang, H. Y.
dc.contributor.authorMakohon-Moore, S. C.
dc.contributor.authorSalhadar, S.
dc.contributor.authorGokhale, R. H.
dc.contributor.authorWarner, R. D.
dc.contributor.authorKoyama, T.
dc.contributor.authorRiddiford, L. M.
dc.contributor.authorShingleton, A. W.
dc.date.accessioned2015-10-06T12:16:18Z
dc.date.available2015-10-06T12:16:18Z
dc.date.issued2014
dc.description.abstractThe role of juvenile hormone (JH) in regulating the timing and nature of insect molts is well-established. Increasing evidence suggests that JH is also involved in regulating final insect size. Here we elucidate the developmental mechanism through which JH regulates body size in developing Drosophila larvae by genetically ablating the JH-producing organ, the corpora allata (CA). We found that larvae that lack CA pupariated at smaller sizes than control larvae due to a reduced larval growth rate. Neither the timing of the metamorphic molt nor the duration of larval growth was affected by the loss of JH. Further, we show that the effects of JH on growth rate are dependent on the forkhead box O transcription factor (FOXO), which is negatively regulated by the insulin-signaling pathway. Larvae that lacked the CA had elevated levels of FOXO activity, whereas a loss-of-function mutation of FOXO rescued the effects of CA ablation on final body size. Finally, the effect of JH on growth appears to be mediated, at least in part, via ecdysone synthesis in the prothoracic gland. These results indicate a role of JH in regulating growth rate via the ecdysone- and insulin-signaling pathways.pt_PT
dc.description.sponsorshipNational Science Foundation Grants: (IOS-0919855, IOS-084584), Howard Hughes Medical Institute, Fundação Calouste Gulbenkian, FCT : [SFRH/Bolsas de Pós-Doutoramento (BPD)/74313/2010].pt_PT
dc.identifier10.1073/pnas.1313058111
dc.identifier.doi10.1073/pnas.1313058111
dc.identifier.urihttp://hdl.handle.net/10400.7/364
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNational Academy of Sciencespt_PT
dc.relation.publisherversionhttp://www.pnas.org/content/111/19/7018.long#cited-bypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectsize controlpt_PT
dc.subjectdevelopmental hormonespt_PT
dc.subjectinsect physiologypt_PT
dc.titleJuvenile hormone regulates body size and perturbs insulin signaling in Drosophilapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7023pt_PT
oaire.citation.issue19pt_PT
oaire.citation.startPage7018pt_PT
oaire.citation.titleProceedings of the National Academy of Sciences of the United States of Americapt_PT
oaire.citation.volume111pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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