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Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis

2015-03-04Journal article Open access
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dc.contributor.authorRaposo, Alexandre A.S.F.
dc.contributor.authorVasconcelos, Francisca F.
dc.contributor.authorDrechsel, Daniela
dc.contributor.authorMarie, Corentine
dc.contributor.authorJohnston, Caroline
dc.contributor.authorDolle, Dirk
dc.contributor.authorBithell, Angela
dc.contributor.authorGillotin, Sébastien
dc.contributor.authorvan den Berg, Debbie L.C.
dc.contributor.authorEttwiller, Laurence
dc.contributor.authorFlicek, Paul
dc.contributor.authorCrawford, Gregory E.
dc.contributor.authorParras, Carlos M.
dc.contributor.authorBerninger, Benedikt
dc.contributor.authorBuckley, Noel J.
dc.contributor.authorGuillemot, François
dc.contributor.authorCastro, Diogo S.
dc.date.accessioned2015-10-21T10:17:49Z
dc.date.available2015-10-21T10:17:49Z
dc.date.issued2015-03-04
dc.description.abstractThe proneural transcription factor Ascl1 coordinates gene expression in both proliferating and differentiating progenitors along the neuronal lineage. Here, we used a cellular model of neurogenesis to investigate how Ascl1 interacts with the chromatin landscape to regulate gene expression when promoting neuronal differentiation. We find that Ascl1 binding occurs mostly at distal enhancers and is associated with activation of gene transcription. Surprisingly, the accessibility of Ascl1 to its binding sites in neural stem/progenitor cells remains largely unchanged throughout their differentiation, as Ascl1 targets regions of both readily accessible and closed chromatin in proliferating cells. Moreover, binding of Ascl1 often precedes an increase in chromatin accessibility and the appearance of new regions of open chromatin, associated with de novo gene expression during differentiation. Our results reveal a function of Ascl1 in promoting chromatin accessibility during neurogenesis, linking the chromatin landscape at Ascl1 target regions with the temporal progression of its transcriptional program.pt_PT
dc.description.sponsorshipFCT doctoral fellowship, FCT investigator program, Marie Curie CIG: (303644), Welcome Trust grants: (WT095908, WT098051).pt_PT
dc.identifier10.1016/j.celrep.2015.02.025
dc.identifier.doi10.1016/j.celrep.2015.02.025
dc.identifier.urihttp://hdl.handle.net/10400.7/418
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCell Presspt_PT
dc.relationCharacterization of a neurogenic program activated by the proneural factor Mash1
dc.relationCis-regulatory logic of the transcriptional control in neural stem cells
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S2211124715001710pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAscl1pt_PT
dc.subjectNeurogenesispt_PT
dc.subjectDNApt_PT
dc.titleAscl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCharacterization of a neurogenic program activated by the proneural factor Mash1
oaire.awardTitleCis-regulatory logic of the transcriptional control in neural stem cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-NEU%2F100208%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FNEU-NMC%2F0315%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/223210/EU
oaire.citation.endPage1556pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1544pt_PT
oaire.citation.titleCell Reportspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication79974431-a97f-457c-8d6f-f1a95439ee0b
relation.isProjectOfPublication80243a1b-138c-4910-968f-415ee9d3a251
relation.isProjectOfPublication028562e4-709a-46d2-a07f-5124bcbc9f53
relation.isProjectOfPublication.latestForDiscovery028562e4-709a-46d2-a07f-5124bcbc9f53

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