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Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation

2017-05-06Journal article Open access
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dc.contributor.authorPiskadlo, Ewa
dc.contributor.authorTavares, Alexandra
dc.contributor.authorOliveira, Raquel A
dc.date.accessioned2018-01-31T13:05:19Z
dc.date.available2018-01-31T13:05:19Z
dc.date.issued2017-05-06
dc.descriptionThis deposit is composed by the main article and the supplementary materials are present in the publisher's page in the following link: https://elifesciences.org/articles/26120/figures.pt_PT
dc.description.abstractMitotic chromosome assembly remains a big mystery in biology. Condensin complexes are pivotal for chromosome architecture yet how they shape mitotic chromatin remains unknown. Using acute inactivation approaches and live-cell imaging in Drosophila embryos, we dissect the role of condensin I in the maintenance of mitotic chromosome structure with unprecedented temporal resolution. Removal of condensin I from pre-established chromosomes results in rapid disassembly of centromeric regions while most chromatin mass undergoes hyper-compaction. This is accompanied by drastic changes in the degree of sister chromatid intertwines. While wild-type metaphase chromosomes display residual levels of catenations, upon timely removal of condensin I, chromosomes present high levels of de novo Topoisomerase II (TopoII)-dependent re-entanglements, and complete failure in chromosome segregation. TopoII is thus capable of re-intertwining previously separated DNA molecules and condensin I continuously required to counteract this erroneous activity. We propose that maintenance of chromosome resolution is a highly dynamic bidirectional process.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia grants: (SRFH/BD/52172/2013, IF/0085½012/CP0185/CT0004 WP1); European Commission grants: (MCCIG321883/CCC, ERC-2014-STG-638917-ChromoCellDev); European Molecular Biology Organization grant: (IG2778).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationeLife 2017;6:e26120 doi: 10.7554/eLife.26120pt_PT
dc.identifier.doi10.7554/eLife.26120pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/826
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publishereLife Sciences Publicationspt_PT
dc.relationIG2778pt_PT
dc.relationIF/0085½012/CP0185/CT0004 WP1pt_PT
dc.relationChromosome Condensation and Cohesion
dc.relationChromosome Architecture and the Fidelity of Mitosis during Development
dc.relation.publisherversionhttps://elifesciences.org/articles/26120pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDrosophila melanogasterpt_PT
dc.subjectcell biologypt_PT
dc.subjectchromosome condensationpt_PT
dc.subjectcondensinpt_PT
dc.subjectmitosispt_PT
dc.subjecttopoisomerasept_PT
dc.titleMetaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleChromosome Condensation and Cohesion
oaire.awardTitleChromosome Architecture and the Fidelity of Mitosis during Development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F52172%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/321883/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/638917/EU
oaire.citation.endPage22pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleeLifept_PT
oaire.citation.volume6pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamFP7
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication60c366d1-cdbe-44ab-a88f-4fb3051f180d
relation.isProjectOfPublicationbc69ed99-dc80-4ac2-80f3-0f5a049f116b
relation.isProjectOfPublicationf620ad03-78b4-4edd-b779-2255668283ed
relation.isProjectOfPublication.latestForDiscovery60c366d1-cdbe-44ab-a88f-4fb3051f180d

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