dc.contributor.author	Jesus, Tiago F.
dc.contributor.author	Moreno, João M.
dc.contributor.author	Repolho, Tiago
dc.contributor.author	Athanasiadis, Alekos
dc.contributor.author	Rosa, Rui
dc.contributor.author	Almeida-Val, Vera M. F.
dc.contributor.author	Coelho, Maria M.
dc.date.accessioned	2017-07-25T16:39:56Z
dc.date.available	2017-07-25T16:39:56Z
dc.date.issued	2017-07-18
dc.description	This deposit is composed by the main article plus the supplementary materials of the publication.	pt_PT
dc.description.abstract	Current knowledge on the biological responses of freshwater fish under projected scenarios of climate change remains limited. Here, we examine differences in the protein configuration of two endemic Iberian freshwater fish species, Squalius carolitertii and the critically endangered S. torgalensis that inhabit in the Atlantic-type northern and in the Mediterranean-type southwestern regions, respectively. We performed protein structure modeling of fourteen genes linked to protein folding, energy metabolism, circadian rhythms and immune responses. Structural differences in proteins between the two species were found for HSC70, FKBP52, HIF1α and GPB1. For S. torgalensis, besides structural differences, we found higher thermostability for two proteins (HSP90 and GBP1), which can be advantageous in a warmer environment. Additionally, we investigated how these species might respond to projected scenarios of 3° climate change warming, acidification (ΔpH = -0.4), and their combined effects. Significant changes in gene expression were observed in response to all treatments, particularly under the combined warming and acidification. While S. carolitertii presented changes in gene expression for multiple proteins related to folding (hsp90aa1, hsc70, fkbp4 and stip1), only one such gene was altered in S. torgalensis (stip1). However, S. torgalensis showed a greater capacity for energy production under both the acidification and combined scenarios by increasing cs gene expression and maintaining ldha gene expression in muscle. Overall, these findings suggest that S. torgalensis is better prepared to cope with projected climate change. Worryingly, under the simulated scenarios, disturbances to circadian rhythm and immune system genes (cry1aa, per1a and gbp1) raise concerns for the persistence of both species, highlighting the need to consider multi-stressor effects when evaluating climate change impacts upon fish. This work also highlights that assessments of the potential of endangered freshwater species to cope with environmental change are crucial to help decision-makers adopt future conservation strategies.	pt_PT
dc.description.sponsorship	Instituto da Conservação da Natureza e Florestas.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Jesus TF, Moreno JM, Repolho T, Athanasiadis A, Rosa R, Almeida-Val VMF, et al. (2017) Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario. PLoS ONE 12(7): e0181325. https://doi.org/10.1371/journal.pone.0181325	pt_PT
dc.identifier.doi	10.1371/journal.pone.0181325	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/779
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Public Library of Science	pt_PT
dc.relation.publisherversion	http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181325	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Gene expression	pt_PT
dc.subject	Climate change	pt_PT
dc.subject	Freshwater fish	pt_PT
dc.subject	Energy metabolism	pt_PT
dc.subject	Heat shock response	pt_PT
dc.subject	Protein folding	pt_PT
dc.subject	Circadian rhythms	pt_PT
dc.subject	Protein structure prediction	pt_PT
dc.title	Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardURI	info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F73801%2F2010/PT
oaire.citation.endPage	18	pt_PT
oaire.citation.issue	7	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	PLoS ONE	pt_PT
oaire.citation.volume	12	pt_PT
oaire.fundingStream	SFRH
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	820b3d3e-aa4d-4d8f-bb0d-a9c174462899
relation.isProjectOfPublication.latestForDiscovery	820b3d3e-aa4d-4d8f-bb0d-a9c174462899

Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario

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