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Wolbachia Variants Induce Differential Protection to Viruses in Drosophila melanogaster: A Phenotypic and Phylogenomic Analysis

2013-12-12Journal article Open access
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dc.contributor.authorChrostek, Ewa
dc.contributor.authorMarialva, Marta S. P.
dc.contributor.authorEsteves, Sara S.
dc.contributor.authorWeinert, Lucy A.
dc.contributor.authorMartinez, Julien
dc.contributor.authorJiggins, Francis M.
dc.contributor.authorTeixeira, Luis
dc.date.accessioned2015-10-26T12:41:28Z
dc.date.available2015-10-26T12:41:28Z
dc.date.issued2013-12-12
dc.description.abstractWolbachia are intracellular bacterial symbionts that are able to protect various insect hosts from viral infections. This tripartite interaction was initially described in Drosophila melanogaster carrying wMel, its natural Wolbachia strain. wMel has been shown to be genetically polymorphic and there has been a recent change in variant frequencies in natural populations. We have compared the antiviral protection conferred by different wMel variants, their titres and influence on host longevity, in a genetically identical D. melanogaster host. The phenotypes cluster the variants into two groups--wMelCS-like and wMel-like. wMelCS-like variants give stronger protection against Drosophila C virus and Flock House virus, reach higher titres and often shorten the host lifespan. We have sequenced and assembled the genomes of these Wolbachia, and shown that the two phenotypic groups are two monophyletic groups. We have also analysed a virulent and over-replicating variant, wMelPop, which protects D. melanogaster even better than the closely related wMelCS. We have found that a ~21 kb region of the genome, encoding eight genes, is amplified seven times in wMelPop and may be the cause of its phenotypes. Our results indicate that the more protective wMelCS-like variants, which sometimes have a cost, were replaced by the less protective but more benign wMel-like variants. This has resulted in a recent reduction in virus resistance in D. melanogaster in natural populations worldwide. Our work helps to understand the natural variation in wMel and its evolutionary dynamics, and inform the use of Wolbachia in arthropod-borne disease control.pt_PT
dc.description.sponsorshipFCT PhD fellowship: (SFRH/BD/51625/2011), Royal Society University Research Fellowship.pt_PT
dc.identifier10.1371/journal.pgen.1003896
dc.identifier.citationChrostek E, Marialva MSP, Esteves SS, Weinert LA, Martinez J, et al. (2013) Wolbachia Variants Induce Differential Protection to Viruses in Drosophila melanogaster : A Phenotypic and Phylogenomic Analysis. PLoS Genet 9(12): e1003896. doi:10.1371/journal.pgen.1003896pt_PT
dc.identifier.doi10.1371/journal.pgen.1003896
dc.identifier.urihttp://hdl.handle.net/10400.7/441
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relationAnalysis of Wolbachia protection against viruses in Drosophila melanogaster
dc.relation.publisherversionhttp://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1003896pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWolbachiapt_PT
dc.subjectDrosophila melanogasterpt_PT
dc.subjectPhenotypept_PT
dc.subjectPhylogenypt_PT
dc.subjectVirus Diseasespt_PT
dc.titleWolbachia Variants Induce Differential Protection to Viruses in Drosophila melanogaster: A Phenotypic and Phylogenomic Analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAnalysis of Wolbachia protection against viruses in Drosophila melanogaster
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-MIC%2F108327%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-MII%2F105655%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/WT/094664
oaire.citation.endPage22pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLOS Geneticspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream094664
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/100010269
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameWellcome Trust
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication7b7988d6-3c39-44df-a97c-2dcbbcdb6bdd
relation.isProjectOfPublicationba0bda37-baec-46f3-ac2b-11b2dcd28e3f
relation.isProjectOfPublication94315fdc-98fe-49ab-ad58-b40bb97543b8
relation.isProjectOfPublication.latestForDiscovery94315fdc-98fe-49ab-ad58-b40bb97543b8

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