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Noninvasive molecular sexing: An evaluation and validation of the SRY- and amelogenin-based method in three new lemur species

2013-03Journal article Open access
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dc.contributor.authorVanpé, Cécile
dc.contributor.authorSalmona, Jordi
dc.contributor.authorPais, Isa
dc.contributor.authorKun-Rodrigues, Célia
dc.contributor.authorPichon, Claire
dc.contributor.authorMeyler, Samuel Viana
dc.contributor.authorRabarivola, Clément
dc.contributor.authorLewis, Rebecca J.
dc.contributor.authorIbouroi, Mohamed Thani
dc.contributor.authorChikhi, Lounès
dc.date.accessioned2016-03-22T13:17:36Z
dc.date.available2016-03-22T13:17:36Z
dc.date.issued2013-03
dc.description.abstractMany lemur species are arboreal, elusive, and/or nocturnal and are consequently difficult to approach, observe and catch. In addition, most of them are endangered. For these reasons, non-invasive sampling is especially useful in primates including lemurs. A key issue in conservation and ecological studies is to identify the sex of the sampled individuals to investigate sex-biased dispersal, parentage, social organization and population sex ratio. Several molecular tests of sex are available in apes and monkeys, but only a handful of them work in the lemuriform clade. Among these tests, the coamplification of the SRY gene with the amelogenin X gene using strepsirhine-specific X primers seems particularly promising, but the reliability and validity of this sexing test have not been properly assessed yet. In this study, we (i) show that this molecular sexing test works on three additional lemur species (Microcebus tavaratra, Propithecus coronatus and P. verreauxi) from two previously untested genera and one previously untested family, suggesting that these markers are likely to be universal among lemurs and other strepsirrhines; (ii) provide the first evidence that this PCR-based sexing test works on degraded DNA obtained from noninvasive samples; (iii) validate the approach using a large number of known-sex individuals and a multiple-tubes approach, and show that mismatches between the field sex and the final molecular consensus sex occur in less than 10% of all the samples and that most of these mismatches were likely linked to incorrect sex determinations in the field rather than genotyping errors.pt_PT
dc.description.sponsorshipFCT fellowship: (SFRH/BPD/73675/2010); Institut Français de la Biodiversité grant: (CD-AOOI-07-003); Programme Biodiversité de l'Océan Indien grant: (CD-AOOI-07-003); Laboratoire d'Excellence (LABEX) entitled TULIP grant: (ANR -10-LABX-41); Instituto Gulbenkian de Ciência- Optimus!Alive-IGC fellowship: (UMR 7206 – CNRS).pt_PT
dc.identifier.citationVanpé, C., Salmona, J., Pais, I., Kun-Rodrigues, C., Pichon, C., Meyler, S. V., Rabarivola, C., Lewis, R. J., Ibouroi, M. T. and Chikhi, L. (2013), Noninvasive molecular sexing: An evaluation and validation of the SRY- and amelogenin-based method in three new lemur species. Am. J. Phys. Anthropol., 150: 492–503. doi: 10.1002/ajpa.22222pt_PT
dc.identifier.doi10.1002/ajpa.22222pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/568
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/ajpa.22222/abstract;jsessionid=D4460F4A72F912035754D0CBD9CD3998.f03t03pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPCR-based sex testpt_PT
dc.subjectnon-invasive samplespt_PT
dc.subjectPropithecuspt_PT
dc.subjectMicrocebuspt_PT
dc.subjectmultiple-tubes approachpt_PT
dc.titleNoninvasive molecular sexing: An evaluation and validation of the SRY- and amelogenin-based method in three new lemur speciespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BEC%2F100176%2F2008/PT
oaire.citation.endPage503pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage492pt_PT
oaire.citation.titleAmerican Journal of Physical Anthropologypt_PT
oaire.citation.volume150pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione097521b-ad8c-4626-a074-aeb1cb70c4bd
relation.isProjectOfPublication.latestForDiscoverye097521b-ad8c-4626-a074-aeb1cb70c4bd

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