Hoxb6 can interfere with somitogenesis in the posterior embryo through a mechanism independent of its rib-promoting activity

Casaca, Ana; Nóvoa, Ana; Mallo, Moisés

Publication

Hoxb6 can interfere with somitogenesis in the posterior embryo through a mechanism independent of its rib-promoting activity

2016-02-01Journal article

dc.contributor.author	Casaca, Ana
dc.contributor.author	Nóvoa, Ana
dc.contributor.author	Mallo, Moisés
dc.date.accessioned	2016-06-14T15:06:40Z
dc.date.available	2016-06-14T15:06:40Z
dc.date.issued	2016-02-01
dc.description.abstract	Formation of the vertebrate axial skeleton requires coordinated Hox gene activity. Hox group 6 genes are involved in the formation of the thoracic area owing to their unique rib-promoting properties. Here we show that the linker region (LR) connecting the homeodomain and the hexapeptide is essential for Hoxb6 rib-promoting activity in mice. The LR-defective Hoxb6 protein was still able to bind a target enhancer together with Pax3, producing a dominant-negative effect, indicating that the LR brings additional regulatory factors to target DNA elements. We also found an unexpected association between Hoxb6 and segmentation in the paraxial mesoderm. In particular, Hoxb6 can disturb somitogenesis and anterior-posterior somite patterning by dysregulation of Lfng expression. Interestingly, this interaction occurred differently in thoracic versus more caudal embryonic areas, indicating functional differences in somitogenesis before and after the trunk-to-tail transition. Our results suggest the requirement of precisely regulated Hoxb6 expression for proper segmentation at tailbud stages.	pt_PT
dc.description.sponsorship	Fundação para a Ciência e a Tecnologia grant: (PTDC/SAU-BID/110640/2009); FCT postdoctoral fellowship: (SFRH/BPD/89500/2012).	pt_PT
dc.identifier.citation	Hoxb6 can interfere with somitogenesis in the posterior embryo through a mechanism independent of its rib-promoting activity Ana Casaca, Ana Nóvoa, Moisés Mallo Development 2016 143: 437-448; doi: 10.1242/dev.133074	pt_PT
dc.identifier.doi	10.1242/dev.133074	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/645
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Company of Biologists	pt_PT
dc.relation.publisherversion	http://dev.biologists.org/content/143/3/437	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Hox genes	pt_PT
dc.subject	Vertebrate patterning	pt_PT
dc.subject	Axial skeleton	pt_PT
dc.subject	Gene regulation	pt_PT
dc.subject	Mouse	pt_PT
dc.title	Hoxb6 can interfere with somitogenesis in the posterior embryo through a mechanism independent of its rib-promoting activity	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	448	pt_PT
oaire.citation.issue	3	pt_PT
oaire.citation.startPage	437	pt_PT
oaire.citation.title	Development (Cambridge, England)	pt_PT
oaire.citation.volume	143	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT