Stage-Specific Plasticity in Ovary Size Is Regulated by Insulin/Insulin-Like Growth Factor and Ecdysone Signaling in Drosophila

Mendes, C. C.; Mirth, C. K.

Publication

Stage-Specific Plasticity in Ovary Size Is Regulated by Insulin/Insulin-Like Growth Factor and Ecdysone Signaling in Drosophila

2016-02-01Journal article

dc.contributor.author	Mendes, C. C.
dc.contributor.author	Mirth, C. K.
dc.date.accessioned	2016-05-17T11:44:56Z
dc.date.available	2017-02-01T01:30:08Z
dc.date.issued	2016-02-01
dc.description.abstract	Animals from flies to humans adjust their development in response to environmental conditions through a series of developmental checkpoints, which alter the sensitivity of organs to environmental perturbation. Despite their importance, we know little about the molecular mechanisms through which this change in sensitivity occurs. Here we identify two phases of sensitivity to larval nutrition that contribute to plasticity in ovariole number, an important determinant of fecundity, in Drosophila melanogaster. These two phases of sensitivity are separated by the developmental checkpoint called "critical weight"; poor nutrition has greater effects on ovariole number in larvae before critical weight than after. We find that this switch in sensitivity results from distinct developmental processes. In precritical weight larvae, poor nutrition delays the onset of terminal filament cell differentiation, the starting point for ovariole development, and strongly suppresses the rate of terminal filament addition and the rate of increase in ovary volume. Conversely, in postcritical weight larvae, poor nutrition affects only the rate of increase in ovary volume. Our results further indicate that two hormonal pathways, the insulin/insulin-like growth factor and the ecdysone-signaling pathways, modulate the timing and rates of all three developmental processes. The change in sensitivity in the ovary results from changes in the relative contribution of each pathway to the rates of terminal filament addition and increase in ovary volume before and after critical weight. Our work deepens our understanding of how hormones act to modify the sensitivity of organs to environmental conditions, thereby affecting their plasticity.	pt_PT
dc.description.sponsorship	NICHD; University of Iowa; FCT fellowship (SFRH/BD/51624/2011); Fundação Calouste Gulbenkian; Instituto Gulbenkian de Ciência.	pt_PT
dc.identifier.citation	Stage-Specific Plasticity in Ovary Size Is Regulated by Insulin/Insulin-Like Growth Factor and Ecdysone Signaling in Drosophila Cláudia C. Mendes, View ORCID ProfileChristen K. Mirth Genetics February 1, 2016 vol. 202 no. 2 703-719; DOI: 10.1534/genetics.115.179960	pt_PT
dc.identifier.doi	10.1534/genetics.115.179960	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/606
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.relation.publisherversion	http://www.genetics.org/content/202/2/703.article-info	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	critical period for environmental sensitivity	pt_PT
dc.subject	ecdysone signalling	pt_PT
dc.subject	insulin/insulin-like growth factor signalling	pt_PT
dc.subject	larval nutrition	pt_PT
dc.subject	ovary size	pt_PT
dc.title	Stage-Specific Plasticity in Ovary Size Is Regulated by Insulin/Insulin-Like Growth Factor and Ecdysone Signaling in Drosophila	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	719	pt_PT
oaire.citation.issue	2	pt_PT
oaire.citation.startPage	703	pt_PT
oaire.citation.title	Genetics	pt_PT
oaire.citation.volume	202	pt_PT
rcaap.rights	embargoedAccess	pt_PT
rcaap.type	article	pt_PT