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Fitness Measurements of Evolved Esherichia coli

2014-09-05Journal article Open access
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dc.contributor.authorMiskinyte, Migla
dc.contributor.authorGordo, Isabel
dc.date.accessioned2015-12-22T14:47:40Z
dc.date.issued2014-09-05
dc.description.abstractBacteria can adapt very rapidly to novel selective pressures. In the transition from commensalism to pathogenicity bacteria have to face and adapt to the host immune system. Specifically, the antagonistic interaction imposed by one of the first line of defense of innate immunity cells, macrophages, on commensal bacteria, such as Escherichia coli (E. coli), can lead to its rapid adaptation. Such adaptation is characterized by the emergence of clones with mutations that allow them to better escape macrophage phagocytosis. Here, we describe how to quantify the amount of fitness increase of bacterial clones that evolved under the constant selective pressure of macrophages, from a murine cell line RAW 264.7. The most widely used assay for measuring fitness changes along an evolutionary laboratory experiment is a competitive fitness assay. This assay consists of determining how fast an evolved strain outcompetes the ancestral in a competition where each starts at equal frequency. The strains compete in the same environment of the evolution experiment and if the evolved strain has acquired strong beneficial mutations it will become significantly overrepresented in repeated competitive fitness assays.pt_PT
dc.description.sponsorshipLAO/ITQB, FCT.pt_PT
dc.identifier.citationMiskinyte, M. and Gordo, I. (2014). Fitness Measurements of Evolved Esherichia coli. Bio-protocol 4(17): e1228. http://www.bio-protocol.org/e1228pt_PT
dc.identifier.otherhttp://www.bio-protocol.org/e1228
dc.identifier.urihttp://hdl.handle.net/10400.7/542
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBio-Protocolpt_PT
dc.relationMicrobial adaptation within ecosystems
dc.relation.publisherversionhttp://www.bio-protocol.org/pdf/20150106105028185/1228%20Fitness%20Measurements%20of%20evolved%20E%20coli%20Tracked%20Changes.pdfpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicrobiologypt_PT
dc.subjectMicrobe-host interactionspt_PT
dc.subjectBacteriumpt_PT
dc.subjectMicrobial geneticspt_PT
dc.subjectDNApt_PT
dc.subjectDNA replicationpt_PT
dc.subjectMolecular Biologypt_PT
dc.subjectGenotypingpt_PT
dc.subjectBacteriapt_PT
dc.subjectEscherichiapt_PT
dc.subjectEscherichia colipt_PT
dc.subjectCell-based analysispt_PT
dc.titleFitness Measurements of Evolved Esherichia colipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicrobial adaptation within ecosystems
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/260421/EU
oaire.citation.endPage5pt_PT
oaire.citation.issue17pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlebio-protocolpt_PT
oaire.citation.volume4pt_PT
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1f01df05-f8af-4e76-8dec-0fc6237b41fd
relation.isProjectOfPublication.latestForDiscovery1f01df05-f8af-4e76-8dec-0fc6237b41fd

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