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The Major Facilitator Superfamily Transporter ZIFL2 Modulates Cesium and Potassium Homeostasis in Arabidopsis

2015-01Journal article Open access
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dc.contributor.authorRemy, E.
dc.contributor.authorCabrito, T. R.
dc.contributor.authorBatista, R. A.
dc.contributor.authorTeixeira, M. C.
dc.contributor.authorSa-Correia, I.
dc.contributor.authorDuque, P.
dc.date.accessioned2016-04-26T17:41:45Z
dc.date.available2016-04-26T17:41:45Z
dc.date.issued2015-01
dc.description.abstractPotassium (K(+)) is an essential mineral nutrient for plant growth and development, with numerous membrane transporters and channels having been implicated in the maintenance and regulation of its homeostasis. The cation cesium (Cs(+)) is toxic for plants but shares similar chemical properties to the K(+) ion and hence competes with its transport. Here, we report that K(+) and Cs(+) homeostasis in Arabidopsis thaliana also requires the action of ZIFL2 (Zinc-Induced Facilitator-Like 2), a member of the Major Facilitator Superfamily (MFS) of membrane transporters. We show that the Arabidopsis ZIFL2 is a functional transporter able to mediate K(+) and Cs(+) influx when heterologously expressed in yeast. Promoter-reporter, reverse transcription-PCR and fluorescent protein fusion experiments indicate that the predominant ZIFL2.1 isoform is targeted to the plasma membrane of endodermal and pericyle root cells. ZIFL2 loss of function and overexpression exacerbate and alleviate plant sensitivity, respectively, upon Cs(+) and excess K(+) supply, also influencing Cs(+) whole-plant partitioning. We propose that the activity of this Arabidopsis MFS carrier promotes cellular K(+) efflux in the root, thereby restricting Cs(+)/K(+) xylem loading and subsequent root to shoot translocation under conditions of Cs(+) or high K(+) external supply.pt_PT
dc.description.sponsorshipFCT fellowships: (SFRH/BPD/44640/2008, SFRH/BPD/81221/2011).pt_PT
dc.identifier.citationEstelle Remy, Tânia R. Cabrito, Rita A. Batista, Miguel C. Teixeira, Isabel Sá-Correia, and Paula Duque The Major Facilitator Superfamily Transporter ZIFL2 Modulates Cesium and Potassium Homeostasis in Arabidopsis Plant Cell Physiol (2015) 56 (1): 148-162 first published online November 4, 2014 doi:10.1093/pcp/pcu157pt_PT
dc.identifier.doi10.1093/pcp/pcu157pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/588
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relation.publisherversionhttp://pcp.oxfordjournals.org/content/56/1/148.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectArabidopsis thalianapt_PT
dc.subjectHeterologous expressionpt_PT
dc.subjectMFS transporterpt_PT
dc.subjectPotassium and cesium homeostasispt_PT
dc.subjectRootpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.titleThe Major Facilitator Superfamily Transporter ZIFL2 Modulates Cesium and Potassium Homeostasis in Arabidopsispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FAGR-PRO%2F1013%2F2013/PT
oaire.citation.endPage162pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage148pt_PT
oaire.citation.titlePlant & Cell Physiologypt_PT
oaire.citation.volume56pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb66a8488-68e7-452c-a018-223ce17fb469
relation.isProjectOfPublication.latestForDiscoveryb66a8488-68e7-452c-a018-223ce17fb469

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