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Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages

2016-01-11Journal article Open access
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dc.contributor.authorAzevedo, M
dc.contributor.authorSousa, A
dc.contributor.authorMoura de Sousa, J
dc.contributor.authorThompson, J A
dc.contributor.authorProença, J T
dc.contributor.authorGordo, I
dc.date.accessioned2016-01-19T17:56:38Z
dc.date.available2016-01-19T17:56:38Z
dc.date.issued2016-01-11
dc.description.abstractThe bacterium Escherichia coli exhibits remarkable genomic and phenotypic variation, with some pathogenic strains having evolved to survive and even replicate in the harsh intra-macrophage environment. The rate and effects of mutations that can cause pathoadaptation are key determinants of the pace at which E. coli can colonize such niches and become pathogenic. We used experimental evolution to determine the speed and evolutionary paths undertaken by a commensal strain of E. coli when adapting to intracellular life. We estimated the acquisition of pathoadaptive mutations at a rate of 10-6 per genome per generation, resulting in the fixation of more virulent strains in less than a hundred generations. Whole genome sequencing of independently evolved clones showed that the main targets of intracellular adaptation involved loss of function mutations in genes implicated in the assembly of the lipopolysaccharide core, iron metabolism and di- and tri-peptide transport, namely rfaI, fhuA and tppB, respectively. We found a substantial amount of antagonistic pleiotropy in evolved populations, as well as metabolic trade-offs, commonly found in intracellular bacteria with reduced genome sizes. Overall, the low levels of clonal interference detected indicate that the first steps of the transition of a commensal E. coli into intracellular pathogens are dominated by a few pathoadaptive mutations with very strong effects.pt_PT
dc.description.sponsorshipLAO/ITQB, FCT.pt_PT
dc.identifier.citationAzevedo M, Sousa A, Moura de Sousa J, Thompson JA, Proença JT, Gordo I (2016) Trade- Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages. PLoS ONE 11(1): e0146123. doi:10.1371/journal.pone.014612pt_PT
dc.identifier.doi10.1371/journal.pone.0146123pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/547
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relationMicrobial adaptation within ecosystems
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146123pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCloningpt_PT
dc.subjectMacrophagespt_PT
dc.subjectEvolutionary adaptationpt_PT
dc.subjectIntracellular pathogenspt_PT
dc.subjectBacterial evolutionpt_PT
dc.subjectBacterial pathogenspt_PT
dc.subjectInsertion mutationpt_PT
dc.subjectBacterial geneticspt_PT
dc.titleTrade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophagespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicrobial adaptation within ecosystems
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/260421/EU
oaire.citation.endPage18pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLOS Onept_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1f01df05-f8af-4e76-8dec-0fc6237b41fd
relation.isProjectOfPublication.latestForDiscovery1f01df05-f8af-4e76-8dec-0fc6237b41fd

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