dc.contributor.author	Brito, Patrícia H
dc.contributor.author	Chevreux, Bastien
dc.contributor.author	Serra, Cláudia R
dc.contributor.author	Schyns, Ghislain
dc.contributor.author	Henriques, Adriano O
dc.contributor.author	Pereira-Leal, José B
dc.date.accessioned	2018-01-24T18:22:10Z
dc.date.available	2018-01-24T18:22:10Z
dc.date.issued	2018-01-20
dc.description	This deposit is composed by the main article plus the supplementary materials of the publication.	pt_PT
dc.description.abstract	Prokaryote genomes are the result of a dynamic flux of genes, with increases achieved via horizontal gene transfer and reductions occurring through gene loss. The ecological and selective forces that drive this genomic flexibility vary across species. Bacillus subtilis is a naturally competent bacterium that occupies various environments, including plant-associated, soil, and marine niches, and the gut of both invertebrates and vertebrates. Here, we quantify the genomic diversity of B. subtilis and infer the genome dynamics that explain the high genetic and phenotypic diversity observed. Phylogenomic and comparative genomic analyses of 42 B. subtilis genomes uncover a remarkable genome diversity that translates into a core genome of 1,659 genes and an asymptotic pangenome growth rate of 57 new genes per new genome added. This diversity is due to a large proportion of low-frequency genes that are acquired from closely related species. We find no gene-loss bias among wild isolates, which explains why the cloud genome, 43% of the species pangenome, represents only a small proportion of each genome. We show that B. subtilis can acquire xenologous copies of core genes that propagate laterally among strains within a niche. While not excluding the contributions of other mechanisms, our results strongly suggest a process of gene acquisition that is largely driven by competence, where the long-term maintenance of acquired genes depends on local and global fitness effects. This competence-driven genomic diversity provides B. subtilis with its generalist character, enabling it to occupy a wide range of ecological niches and cycle through them.	pt_PT
dc.description.sponsorship	Fundação para a Ciência e a Tecnologia grants: (PTDC/EBB-BIO/119006/2010, PEst-OE/EQB/LA0004/2011, SFRH/BPD/89907/2012, SFRH/BD/29397/06); FEDER grant: (LISBOA-01-0145-FEDER-007660).	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Patrícia H Brito, Bastien Chevreux, Cláudia R Serra, Ghislain Schyns, Adriano O Henriques, José B Pereira-Leal; Genetic Competence Drives Genome Diversity in Bacillus subtilis, Genome Biology and Evolution, Volume 10, Issue 1, 1 January 2018, Pages 108–124, https://doi.org/10.1093/gbe/evx270	pt_PT
dc.identifier.doi	10.1093/gbe/evx270	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/822
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Oxford University Press	pt_PT
dc.relation	LISBOA-01-0145-FEDER-007660	pt_PT
dc.relation	Perda de robustez como um princípio de reposicionamento de fármacos
dc.relation	Strategic Project - LA 4 - 2011-2012
dc.relation	EXTRA- AND INTRACELLULAR SIGNALING GOVERNING SPORULATION INITIATION IN UNDOMESTICATED BACILLUS SUBTILIS
dc.relation.publisherversion	https://academic.oup.com/gbe/article/10/1/108/4767717	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	pt_PT
dc.subject	Bacillus subtilis	pt_PT
dc.subject	pangenome	pt_PT
dc.subject	comparative genomics	pt_PT
dc.subject	bacterial genome evolution	pt_PT
dc.subject	lateral gene transfer	pt_PT
dc.subject	genetic competence	pt_PT
dc.title	Genetic Competence Drives Genome Diversity in Bacillus subtilis	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardTitle	Perda de robustez como um princípio de reposicionamento de fármacos
oaire.awardTitle	Strategic Project - LA 4 - 2011-2012
oaire.awardTitle	EXTRA- AND INTRACELLULAR SIGNALING GOVERNING SPORULATION INITIATION IN UNDOMESTICATED BACILLUS SUBTILIS
oaire.awardURI	info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-BIO%2F119006%2F2010/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0004%2F2011/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F89907%2F2012/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F29397%2F2006/PT
oaire.citation.endPage	124	pt_PT
oaire.citation.issue	1	pt_PT
oaire.citation.startPage	108	pt_PT
oaire.citation.title	Genome Biology and Evolution	pt_PT
oaire.citation.volume	10	pt_PT
oaire.fundingStream	3599-PPCDT
oaire.fundingStream	6817 - DCRRNI ID
oaire.fundingStream	SFRH
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	38d12f4c-99a0-4d41-a564-c1a2b7f52311
relation.isProjectOfPublication	75d288a4-f20d-4d22-b743-db7abca71541
relation.isProjectOfPublication	6d508ef0-80f3-4d5f-b0da-9d68ae2b632c
relation.isProjectOfPublication	00e19927-fc29-4762-b83b-4e78696c05e6
relation.isProjectOfPublication.latestForDiscovery	6d508ef0-80f3-4d5f-b0da-9d68ae2b632c

Genetic Competence Drives Genome Diversity in Bacillus subtilis

Files

Original bundle

License bundle

Collections