Compartment-dependent activities of Wnt3a/β-catenin signaling during vertebrate axial extension

Jurberg, Arnon Dias; Aires, Rita; Nóvoa, Ana; Rowland, Jennifer Elizabeth; Mallo, Moisés

Publication

Compartment-dependent activities of Wnt3a/β-catenin signaling during vertebrate axial extension

2014-10-15Journal article

dc.contributor.author	Jurberg, Arnon Dias
dc.contributor.author	Aires, Rita
dc.contributor.author	Nóvoa, Ana
dc.contributor.author	Rowland, Jennifer Elizabeth
dc.contributor.author	Mallo, Moisés
dc.date.accessioned	2016-06-29T12:22:50Z
dc.date.available	2016-06-29T12:22:50Z
dc.date.issued	2014-10-15
dc.description.abstract	Extension of the vertebrate body results from the concerted activity of many signals in the posterior embryonic end. Among them, Wnt3a has been shown to play relevant roles in the regulation of axial progenitor activity, mesoderm formation and somitogenesis. However, its impact on axial growth remains to be fully understood. Using a transgenic approach in the mouse, we found that the effect of Wnt3a signaling varies depending on the target tissue. High levels of Wnt3a in the epiblast prevented formation of neural tissues, but did not impair axial progenitors from producing different mesodermal lineages. These mesodermal tissues maintained a remarkable degree of organization, even within a severely malformed embryo. However, from the cells that failed to take a neural fate, only those that left the epithelial layer of the epiblast activated a mesodermal program. The remaining tissue accumulated as a folded epithelium that kept some epiblast-like characteristics. Together with previously published observations, our results suggest a dose-dependent role for Wnt3a in regulating the balance between renewal and selection of differentiation fates of axial progenitors in the epiblast. In the paraxial mesoderm, appropriate regulation of Wnt/β-catenin signaling was required not only for somitogenesis, but also for providing proper anterior-posterior polarity to the somites. Both processes seem to rely on mechanisms with different requirements for feedback modulation of Wnt/β-catenin signaling, once segmentation occurred in the presence of high levels of Wnt3a in the presomitic mesoderm, but not after permanent expression of a constitutively active form of β-catenin. Together, our findings suggest that Wnt3a/β-catenin signaling plays sequential roles during posterior extension, which are strongly dependent on the target tissue. This provides an additional example of how much the functional output of signaling systems depends on the competence of the responding cells.	pt_PT
dc.description.sponsorship	Fundação para a Ciência e a Tecnologia: (PTDC/BIA-BCM/110638/2009, PTDC/SAU-BID/110640/2009, SFRH/BD/33562/2008, SFRH/BD/51876/2012).	pt_PT
dc.identifier.citation	Arnon Dias Jurberg, Rita Aires, Ana Nóvoa, Jennifer Elizabeth Rowland, Moisés Mallo, Compartment-dependent activities of Wnt3a/β-catenin signaling during vertebrate axial extension, Developmental Biology, Volume 394, Issue 2, 15 October 2014, Pages 253-263, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2014.08.012. (http://www.sciencedirect.com/science/article/pii/S0012160614004035) Keywords: Wnt signaling; Axial progenitors; Patterning; Mouse development	pt_PT
dc.identifier.doi	10.1016/j.ydbio.2014.08.012	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/674
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Elsivier Science BV	pt_PT
dc.relation.publisherversion	http://www.sciencedirect.com/science/article/pii/S0012160614004035	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Animals	pt_PT
dc.subject	Body Patterning	pt_PT
dc.subject	Cell Differentiation	pt_PT
dc.subject	Mice	pt_PT
dc.subject	Mice, Transgenic	pt_PT
dc.subject	Microscopy, Confocal	pt_PT
dc.subject	Signal Transduction	pt_PT
dc.subject	Wnt3A Protein	pt_PT
dc.subject	beta Catenin	pt_PT
dc.title	Compartment-dependent activities of Wnt3a/β-catenin signaling during vertebrate axial extension	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	263	pt_PT
oaire.citation.issue	2	pt_PT
oaire.citation.startPage	253	pt_PT
oaire.citation.title	Developmental Biology	pt_PT
oaire.citation.volume	394	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT