Spontaneous telomere to telomere fusions occur in unperturbed fission yeast cells

Almeida, H.; Godinho Ferreira, M.

http://hdl.handle.net/10400.7/422

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Authors

Almeida, H.

Godinho Ferreira, M.

Abstract(s)

Telomeres protect eukaryotic chromosomes from illegitimate end-to-end fusions. When this function fails, dicentric chromosomes are formed, triggering breakage-fusion-bridge cycles and genome instability. How efficient is this protection mechanism in normal cells is not fully understood. We created a positive selection assay aimed at capturing chromosome-end fusions in Schizosaccharomyces pombe. We placed telomere sequences with a head to head arrangement in an intron of a selectable marker contained on a plasmid. By linearizing the plasmid between the telomere sequences, we generated a stable mini-chromosome that fails to express the reporter gene. Whenever the ends of the mini-chromosome join, the marker gene is reconstituted and fusions are captured by direct selection. Using telomerase mutants, we recovered several fusion events that lacked telomere sequences. The end-joining reaction involved specific homologous subtelomeric sequences capable of forming hairpins, suggestive of ssDNA stabilization prior to fusing. These events occurred via microhomology-mediated end-joining (MMEJ)/single-strand annealing (SSA) repair and also required MRN/Ctp1. Strikingly, we were able to capture spontaneous telomere-to-telomere fusions in unperturbed cells. Similar to disruption of the telomere regulator Taz1/TRF2, end-joining reactions occurred via non-homologous end-joining (NHEJ) repair. Thus, telomeres undergo fusions prior to becoming critically short, possibly through transient deprotection. These dysfunction events induce chromosome instability and may underlie early tumourigenesis.

Keywords

Telomere Cells

URI

http://hdl.handle.net/10400.7/422

Citation

Hugo Almeida and Miguel Godinho Ferreira Spontaneous telomere to telomere fusions occur in unperturbed fission yeast cells Nucl. Acids Res. (2013) 41 (5): 3056-3067 first published online January 18, 2013 doi:10.1093/nar/gks1459